Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.3K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.3K
The Tumor Microenvironment02:17

The Tumor Microenvironment

2.6K
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Laparoscopic Roux-en-Y Duodenojejunostomy for Duodenal Obstruction after Endovascular Aneurysm Repair Mimicking Superior Mesenteric Artery Syndrome: A Case Report.

Surgical case reports·2026
Same author

Strategic pathways of digital health platforms in regulated markets: Evidence from wearable devices.

Digital health·2026
Same author

Feasibility and safety of surgical strategy based on tumor characteristics and location in laparoscopic and endoscopic cooperative surgery for non-ampullary duodenal tumors.

Updates in surgery·2026
Same author

Mitochondrial inhibition enhances the sensitivity of pancreatic ductal adenocarcinoma cells to oncolytic adenovirus.

Molecular therapy. Oncology·2026
Same author

P53-armed Oncolytic Adenovirus Enhances the Efficacy of PD-1 Blockade in Neuroblastoma by Inducing Immunogenic Cell Death.

Anticancer research·2026
Same author

Risk Factors for Anastomotic Stricture and Obstructive Symptoms Following Double-Flap Technique Reconstruction After Proximal Gastrectomy.

Annals of gastroenterological surgery·2026

Related Experiment Video

Updated: Apr 28, 2026

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

2.2K

Multi-Transcriptomic Analysis Reveals That EREG-Driven TME Crosstalk Defines Anti-EGFR Response in Colorectal Cancer.

Atsuki Taniguchi1, Shunsuke Kagawa1, Shohei Nogi1

  • 1Department of Gastroenterological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Cancer Medicine
|April 27, 2026
PubMed
Summary

Tumor microenvironment features, not just sidedness, predict colorectal cancer (CRC) response to EGFR inhibitors (EGFRI). A key EREG/EGFR/CSF axis involving cancer cells, fibroblasts, and macrophages drives EGFRI eligibility.

Keywords:
EGFR inhibitor eligibilityEpiregulin (EREG)cell–cell interactioncolorectal cancertumor microenvironment

More Related Videos

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
10:33

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

10.3K
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
10:54

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Published on: April 4, 2018

18.2K

Related Experiment Videos

Last Updated: Apr 28, 2026

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

2.2K
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
10:33

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

10.3K
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
10:54

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Published on: April 4, 2018

18.2K

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Tumor sidedness impacts colorectal cancer (CRC) prognosis and EGFR inhibitor (EGFRI) response.
  • The precise mechanisms underlying differential EGFRI sensitivity remain unclear.

Purpose of the Study:

  • To investigate the tumor microenvironment (TME) in relation to EGFRI eligibility in CRC.
  • To identify key cellular interactions and molecular pathways influencing EGFRI response.

Main Methods:

  • Integrated single-cell RNA sequencing (scRNA-seq) for TME analysis.
  • Bulk RNA-seq and spatial transcriptomics for validation.
  • Analysis of cell-cell interactions and pathway activation.

Main Results:

  • Cancer cell features, particularly high Epiregulin (EREG) expression, strongly predicted EGFRI eligibility.
  • Identified an "EREG/EGFR/CSF axis" involving cancer cells, EGFR-expressing CAFs, and TAM/TAMo.
  • Spatial analysis confirmed localized EGFR pathway activation and proximity of interacting cells in eligible tumors.

Conclusions:

  • TME characteristics, specifically the EREG/EGFR/CSF axis, are critical determinants of EGFRI eligibility in CRC.
  • TME-based patient stratification may offer improved precision over sidedness alone for CRC therapy.