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Phytochemical Profiling, Gas Chromatography-Mass Spectrometry Analysis, and In Vivo Activity of Terminalia mantaly in
Farzana Khan1, Samina Afzal2, Hafiz Muhammad Usman Abid3
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bahauddin Zakariya University.
Journal of Visualized Experiments : Jove
|April 27, 2026
Summary
Terminalia mantaly extract shows significant anti-inflammatory, analgesic, and antipyretic effects in rats, validating its traditional use. This study establishes a framework for evaluating medicinal plants, supporting natural drug development.
Area of Science:
- Pharmacology
- Ethnobotany
- Phytochemistry
Background:
- Synthetic anti-inflammatory, analgesic, and antipyretic drugs cause adverse effects.
- Plant-derived alternatives are gaining interest.
- Terminalia mantaly is traditionally used in African medicine but lacks scientific validation.
Purpose of the Study:
- To evaluate the therapeutic potential of the methanolic extract of Terminalia mantaly.
- To validate its ethnomedicinal use through an integrated experimental protocol.
- To link phytochemical composition with pharmacological activity.
Main Methods:
- Phytochemical screening and quantitative analysis of phenolic and flavonoid compounds.
- Gas chromatography-mass spectrometry (GC-MS) for constituent identification.
- In vivo pharmacological testing in rats (paw edema, tail immersion, pyrexia models) and toxicity assessments.
Main Results:
- GC-MS identified 20 constituents, including fatty acids, phytosterols, and tocopherols.
- The extract demonstrated dose-dependent anti-inflammatory, analgesic, and antipyretic activities.
- Effects at higher doses were comparable to diclofenac and paracetamol, with a favorable safety profile.
Conclusions:
- The findings validate the ethnomedicinal use of Terminalia mantaly.
- The study presents a reproducible framework for medicinal plant research.
- Terminalia mantaly extract shows potential for managing inflammatory and febrile conditions.
