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Related Experiment Video

Updated: Apr 29, 2026

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

Investigating Glycolysis in Primary Microglia Using Extracellular Flux Assay.

Aysika Das¹, Deepak K Kaushik²

¹Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland.

Journal of Visualized Experiments : Jove

|April 27, 2026

Summary

This summary is machine-generated.

This study details a method to measure microglial glycolysis, crucial for understanding brain inflammation. The protocol uses a glycolysis stress test to analyze metabolic changes in microglia under various conditions.

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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

Published on: November 21, 2016

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Last Updated: Apr 29, 2026

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

Author Spotlight: Advancing Understanding Through Technological Innovations in Psychoneuroimmunology

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An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

Published on: November 21, 2016

Area of Science:

Neuroscience
Immunology
Cellular Metabolism

Background:

Microglia are key immune cells in the central nervous system.
Their metabolic reprogramming influences inflammatory responses.
Understanding microglial metabolism is vital for neuroinflammation research.

Purpose of the Study:

To establish a protocol for assessing microglial glycolysis.
To investigate metabolic alterations in microglia in response to stimuli.
To provide insights into microglial function during inflammation.

Main Methods:

Isolation of primary microglia from neonatal mouse brain cortices.
Utilizing a glycolysis stress test on an extracellular flux analyzer.
Real-time measurement of extracellular acidification rate (ECAR) to assess glycolytic activity.
Treatment of microglia with various stimuli, including pro-inflammatory agents.
Preparation of fresh reagent stock solutions with precise pH adjustment.

Main Results:

The protocol enables real-time measurement of microglial glycolytic profiles.
Metabolic pathway alterations can be observed in response to different stimuli.
The method allows for detailed analysis of glycolysis in primary microglia.

Conclusions:

This robust protocol facilitates the study of glycolysis in primary microglia.
It offers valuable insights into microglial functional states under inflammatory conditions.
The method aids in understanding the role of microglial metabolism in neuroinflammation.