Related Experiment Video
Updated: May 13, 2026

Creating Dynamic Images of Short-lived Dopamine Fluctuations with lp-ntPET: Dopamine Movies of Cigarette Smoking
Published on: August 6, 2013
N-pyrrolidino isotonitazene and its metabolites in post-mortem casework
1Department of Toxicology, University Hospitals Sussex NHS Foundation Trust, Royal Sussex County Hospital, Eastern Road, Brighton, BN2 5BE, United Kingdom.
None:
N-pyrrolidino isotonitazene (Isotonitazepyne) is an extremely potent novel synthetic opioid which has been detected internationally in counterfeit pharmaceutical tablets. Published data from post-mortem cases is extremely limited and no metabolite profiles from authentic case work have been published. This paper describes a number of post-mortem cases in which N-pyrrolidino isotonitazene was thought to play a significant role in the cause of death, as well as describing the detected metabolite profiles. Concentration ranges for N-pyrrolidino isotonitazene was reported to be 1.1-55.9 µg/L across the 4 cases. The presumptive metabolite 5-amino-N-pyrrolidino isotonitazene was also identified in all samples; n-pyrrolidino-4-hydroxy nitazene was present in 3 of the 4 urine samples; and N-pyrrolidino hydroxy isotonitazene was present in 1 sample. This study highlights some of the challenges associated with nitazene interpretation, such as the very wide concentration ranges encountered in potentially fatal cases, as well as providing provisional metabolite data which may aid in detecting future cases.
Related Concept Videos
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
2° Amines to N-Nitrosamines: Reaction with NaNO2
Physical Properties of Amines
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase

