Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

5.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.0K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

7.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.6K
Export of Misfolded Proteins out of the ER01:32

Export of Misfolded Proteins out of the ER

4.3K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
4.3K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.8K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.8K
Maturation of Endosomes01:28

Maturation of Endosomes

5.1K
The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
5.1K
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

87
Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
87

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ER discontinuities are common in <i>C. elegans</i> neurons, revealing a genetically tractable model for ER network maintenance.

bioRxiv : the preprint server for biology·2026
Same author

The history and evolution of pediatric ophthalmology training: celebrating 50 years of the American Association for Pediatric Ophthalmology and Strabismus.

Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus·2026
Same author

ER remodelling is a feature of ageing and depends on ER-phagy.

Nature cell biology·2026
Same author

Functionally diversified Caenorhabditis elegans BiP orthologs control body growth, reproduction, stress resistance, aging, and autophagy.

Nature communications·2025
Same author

InsP3R signaling mediates mitochondrial stress-induced longevity through actomyosin-dependent mitochondrial dynamics.

bioRxiv : the preprint server for biology·2025
Same author

Nutrient-Responsive Formation of Mitochondrial-Derived Structures in <i>Caenorhabditis elegans</i>.

bioRxiv : the preprint server for biology·2025
Same journal

AARS2-mediated lactylation of ULK1 promotes autophagy-dependent progression of clear cell renal cell carcinoma.

Autophagy·2026
Same journal

Mechanistic studies of autophagic cargo recruitment and membrane shaping through in vitro reconstitution.

Autophagy·2026
Same journal

Receptor - cargo coupling during ER-autophagy depends on coat proteins and ER membrane properties.

Autophagy·2026
Same journal

Translation control of autophagy genes modulates cellular response to hydroxyurea-induced genotoxic stress.

Autophagy·2026
Same journal

RAPSN/rapsyn aggregation-induced HSPA/HSP70-BAG3 aggrephagy maintains CHRN integrity in myasthenia gravis.

Autophagy·2026
Same journal

TMEM184A-mediated autophagy in MHC-I degradation promotes tumor immune evasion.

Autophagy·2026
See all related articles

Related Experiment Video

Updated: Apr 29, 2026

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

5.7K

Autophagy reshapes the aging ER.

Eric K F Donahue1, Kristopher Burkewitz1

  • 1Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN, USA.

Autophagy
|April 28, 2026
PubMed
Summary
This summary is machine-generated.

Selective ER-phagy actively remodels the endoplasmic reticulum (ER) during aging, reducing its volume and altering its structure. This process is crucial for lifespan extension and adaptive cellular metabolism.

Keywords:
ER-phagyEndoplasmic reticulummTORprotein homeostasisunfolded protein response

More Related Videos

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

10.0K
Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K

Related Experiment Videos

Last Updated: Apr 29, 2026

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
06:18

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Published on: April 13, 2018

5.7K
In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

10.0K
Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy
07:20

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

1.4K

Area of Science:

  • Cellular Biology
  • Aging Research
  • Autophagy

Background:

  • Organelle structural changes during aging are often considered passive deterioration.
  • The endoplasmic reticulum (ER) undergoes significant morphological and compositional alterations with age.

Purpose of the Study:

  • To investigate the active mechanisms driving age-associated ER remodeling.
  • To determine the role of ER-phagy in aging and longevity.

Main Methods:

  • Comparative analysis of ER structure and composition across different cell types and organisms.
  • Investigation of ER-phagy pathways and their regulation.
  • Genetic manipulation to assess the requirement of ER-phagy for lifespan extension.

Main Results:

  • Identified an evolutionarily conserved, age-onset ER remodeling driven by selective ER-phagy.
  • Observed ER volume reduction and shift from sheets to tubular networks.
  • Demonstrated that ER-phagy is required for lifespan extension during mechanistic target of rapamycin (mTOR) impairment.

Conclusions:

  • ER-phagy is an active, conserved process regulating ER architecture and cellular metabolism during aging.
  • Selective autophagy plays a vital role in maintaining organelle identity and function across the lifespan.
  • ER turnover via ER-phagy is an adaptive mechanism contributing to longevity.