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Related Concept Videos

Higher Mental Functions of the Brain: Language01:10

Higher Mental Functions of the Brain: Language

Language is a system of communication that allows the expression of thoughts, ideas, and feelings. The brain processes language in both hemispheres.
Language formation and comprehension take place in the dominant hemisphere. The dominant hemisphere is responsible for understanding the meaning of spoken, written, or sign language, as well as the ability to communicate. For most people, the left hemisphere is the dominant one. The right hemisphere, then, gives tone and emotional context to the...
Cerebral Hemispheres01:05

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The human brain, a complex organ, is functionally divided into two cerebral hemispheres—left and right. These hemispheres are interconnected by a structure of paramount importance, the corpus callosum. This substantial bundle of neural fibers is not just a bridge between the hemispheres but a crucial element for the brain's comprehensive functioning. It enables efficient communication between the two hemispheres, allowing each side of the brain to control and receive sensory and motor...
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Brain lateralization refers to the division of mental processes and functions between the two hemispheres of the brain, a phenomenon that optimizes neural efficiency and underpins complex abilities in humans. This specialization allows each hemisphere to perform tasks where it has a comparative advantage, facilitating more refined cognitive capabilities across different domains.
Language and Cognition01:27

Language and Cognition

Language serves as a bridge between ideas and communication, influencing how individuals perceive and interact with the world. Psychologists have long debated whether language shapes thought or vice versa. This discussion gained grip with Edward Sapir and Benjamin Lee Whorf in the 1940s, who proposed that language determines thought, a concept known as linguistic determinism. They suggested that the vocabulary and structure of a language influence how its speakers think and perceive reality.
Learning Disabilities01:25

Learning Disabilities

Learning disabilities are cognitive disorders caused by neurological impairments that affect cognitive functions like language and reading, without indicating overall intellectual or developmental challenges. These disabilities differ from global intellectual or developmental disabilities as they are limited to distinct cognitive functions. Common learning disabilities include dysgraphia, dyslexia, and dyscalculia, each of which impacts unique aspects of learning.
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High-Throughput Single-Cell-Resolved Spatial Proteomics Enabled by an Ordered Colloidal Crystal Column.

Haofei Sun1,2, Chao Wang1,2, Kun Guo3

  • 1State Key Laboratory of Medical Proteomics, National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.

Angewandte Chemie (International Ed. in English)
|April 28, 2026
PubMed
Summary
This summary is machine-generated.

A novel colloidal crystal column significantly accelerates spatial proteomics by enabling rapid nanoLC-MS analysis of microdissected tissue slices. This breakthrough allows for high-throughput proteomic profiling down to single-cell resolution, enhancing biological discovery.

Keywords:
colloidal crystalhigh‐throughputliquid chromatographysingle cellspatial proteomics

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Spatial proteomics is crucial for understanding biological processes and disease.
  • Current nanoLC-MS methods for spatial proteomics are limited by low throughput due to lengthy analysis times.

Purpose of the Study:

  • To develop a high-throughput method for spatial proteomics.
  • To enhance the speed and efficiency of nanoLC-MS analysis for microdissected samples.

Main Methods:

  • Development of an ordered colloidal crystal column for fast nanoLC-MS.
  • Utilizing the column for analysis of microdissected tissue slices, including single-cell resolution.
  • Optimization of gradient times for rapid peptide separation and protein identification.

Main Results:

  • The colloidal crystal column achieved high efficiency (2,560,000 plates·m⁻¹), 10-fold higher than conventional columns.
  • High-throughput analysis identified 5942 and 4388 proteins from HeLa digests using 5- and 2-min gradients, respectively.
  • Exceptional single-cell spatial proteomics identified up to 2304 proteins from a single hepatocyte within a 5-min gradient, 16x faster than conventional methods.

Conclusions:

  • The developed colloidal crystal column significantly boosts throughput for spatial proteomics.
  • This technology enables rapid, high-confidence protein identification at single-cell resolution.
  • The column shows great promise for advancing high-throughput spatial proteomics research.