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Updated: Apr 29, 2026
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Preparing a Mice Model of Severe Acute Pancreatitis via a Combination of Caerulein and Lipopolysaccharide Intraperitoneal Injection
Published on: May 10, 2024
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Using the Phillygenin Ameliorates the Severe Acute Pancreatitis in Rats by Inhibiting TLR4/NF-κB Pathway
Yiwen Sun1,2, Shengze Li1,2, Jiming Duan1
1Department of General Surgery, The Second Hospital of Shanxi Medical University, Taiyuan, People's Republic of China.
Journal of Inflammation Research
|April 28, 2026
Summary
Phillygenin (PHI) effectively treats severe acute pancreatitis (SAP) by reducing inflammation. This study shows PHI inhibits the TLR4/NF-κB pathway, suggesting its potential as a novel therapeutic agent for SAP.
Area of Science:
- Biochemistry
- Pharmacology
- Immunology
Background:
- Severe acute pancreatitis (SAP) lacks effective anti-inflammatory treatments.
- Phillygenin (PHI) demonstrates anti-inflammatory properties, but its mechanism in SAP is unknown.
- This study investigates PHI's therapeutic effects and regulatory mechanisms in SAP.
Purpose of the Study:
- To explore the therapeutic effects of Phillygenin (PHI) on severe acute pancreatitis (SAP).
- To elucidate the molecular mechanisms underlying PHI's action in SAP, focusing on the TLR4/NF-κB pathway.
Main Methods:
- Animal model: Thirty male SD rats divided into control, SAP, and PHI-treated groups.
- In vitro model: Rat pancreatic AR42J cells treated with cerulein/LPS and PHI.
- Assays: Histological scores, serum amylase/lipase, MPO activity, ELISA for cytokines (TNF-α, IL-6, IL-1β), Western blot, and immunohistochemistry for TLR4/NF-κB.
- Molecular docking predicted PHI's affinity to TLR4/NF-κB.
Main Results:
- PHI treatment significantly improved pancreatic histology and reduced serum amylase and lipase levels.
- PHI decreased pancreatic MPO activity and suppressed the release of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β).
- PHI demonstrated binding to TLR4/NF-κB, downregulating their protein expression and nuclear translocation in both in vivo and in vitro models.
Conclusions:
- Phillygenin (PHI) exerts a protective effect against severe acute pancreatitis (SAP).
- PHI alleviates SAP by inhibiting the TLR4/NF-κB signaling pathway.
- PHI shows potential as a novel therapeutic agent for treating SAP.
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