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Related Experiment Video

Updated: Apr 29, 2026

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

Transcriptomic characterization of Coccidioides morphological states using RiboMarker-enhanced RNA sequencing.

Jonathan M Howard¹, Aidan C Manning¹, Rachel C Clark¹

¹RealSeq Biosciences, Santa Cruz, 95060, USA.

G3 (Bethesda, Md.)

|April 28, 2026

Summary

This summary is machine-generated.

This study reveals small RNAs (sRNAs) and RNA fragments in Coccidioides posadasii, the fungus causing Valley fever. These molecules are crucial for fungal life cycle transitions and may facilitate host-pathogen communication.

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Area of Science:

Mycology
Molecular Biology
Pathogenomics

Background:

Coccidioides causes Valley fever, a significant mammalian disease.
Small RNAs (sRNAs) regulate virulence in pathogenic fungi but are unexplored in Coccidioides.
Understanding Coccidioides life cycle transitions is key to controlling infections.

Purpose of the Study:

To profile small RNA (sRNA) and RNA fragment expression across Coccidioides posadasii life stages.
To investigate intracellular and extracellular RNA fractions during morphological transitions.
To identify novel RNA species involved in Coccidioides virulence and host interaction.

Main Methods:

Small RNA sequencing using RiboMarker®️ for enhanced transcriptome coverage.
Analysis of arthroconidia, mycelia, and spherule morphologies.
Comparison of intracellular and extracellular RNA pools.

Main Results:

Significant transcriptomic remodeling observed during arthroconidia-to-mycelia/spherule transitions.
Identification of sRNA and fragment-producing loci potentially involved in life cycle progression.
Distinct RNA fragmentation patterns linked to specific morphological states.
Detection of extracellular RNA export, including snRNAs and tRNA fragments, suggesting communication roles.

Conclusions:

An atlas of intra- and extracellular Coccidioides sRNAs and fragments was generated.
These findings provide insights into Coccidioides virulence and life cycle regulation.
Identified RNA species offer potential for Valley fever biomarker discovery.