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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Apr 30, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

DNA Nanoarray for Multiplexed RNA Detection With Single-Molecule Readout.

Yunxuan Li¹, Yesheng Wang², Chunmiao Yu²

¹Cavendish Laboratory, University of Cambridge, Cambridge, UK.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|April 28, 2026

Summary

This summary is machine-generated.

We developed a novel DNA carrier-based nanoarray for sensitive, single-molecule RNA detection. This platform enables high-throughput molecular diagnostics by combining multiplexing with nanopore precision.

Keywords:

DNA nanotechnology multiplexed molecular detection programmable nanoarrays single‐molecule sensing solid‐state nanopores

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Last Updated: Apr 30, 2026

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

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Published on: August 12, 2019

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Published on: November 15, 2017

Area of Science:

Molecular Diagnostics
Nanotechnology
Biotechnology

Background:

Sensitive and multiplexed RNA analysis at the single-molecule level is crucial for molecular diagnostics.
Current methods like fluorescence microarrays offer high throughput but lack resolution, while nanopore sensors provide precision but limited scalability.

Purpose of the Study:

To present a DNA carrier-based nanoarray for direct, multiplexed RNA detection.
To integrate programmable nucleic acid hybridization with solid-state nanopore readout for enhanced RNA analysis.

Main Methods:

A DNA carrier-based nanoarray with pre-assembled, spatially defined probe sites for sequence-specific RNA capture.
Utilizing poly(dT) sequences to facilitate target binding and enhance signal strength without labeling.
Employing solid-state nanopore translocation to generate ionic current signatures for single-molecule identification.

Main Results:

Successfully constructed a nanoarray with 27 carriers and 81 addressable sensing sites using a ternary coordinate encoding system.
Achieved simultaneous detection of multiple bacterial and viral RNA targets directly from total RNA extracts.
Demonstrated single-molecule identification of target occupancy without target-specific isolation, amplification, or enrichment.

Conclusions:

The carrier-based nanoarray combines the multiplexing of microarrays with the single-molecule precision of nanopore sensing.
This scalable, programmable, and adaptable framework facilitates high-throughput molecular diagnostics in complex biological samples.
The developed platform offers a promising solution for advanced molecular diagnostics and research applications.