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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jun 29, 2026

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
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Charge-Based Discrimination of Amyloids Using an Amyloid-Targeting Chemiluminescent Probe.

Jeewon Chung1, R Erin Jessup1, Jerry Yang1

  • 1Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, California, La Jolla, USA.

Chembiochem : a European Journal of Chemical Biology
|April 28, 2026
PubMed
Summary

A new chemiluminescent probe, CLIP-1, detects amyloid biomarkers like amyloid-beta and tau in neurodegenerative diseases. This probe offers a promising alternative to fluorescent probes for in vivo imaging and diagnosis.

Keywords:
amyloid beta-peptideschemiluminescenceimaging agents

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Detecting Amyloid-β Accumulation via Immunofluorescent Staining in a Mouse Model of Alzheimer's Disease
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Area of Science:

  • Biochemistry
  • Neuroscience
  • Chemical Biology

Background:

  • Fluorescent probes are common for detecting neurodegenerative disease biomarkers but require external light.
  • Limitations of fluorescent probes hinder research and in vivo applications.
  • Chemiluminescent probes offer an alternative for optical imaging by using chemical reactions for light generation.

Purpose of the Study:

  • To design and synthesize a novel chemiluminescent probe, CLIP-1.
  • To evaluate CLIP-1's ability to detect amyloid-beta (Aβ), α-synuclein (α-syn), and tau aggregates.
  • To explore CLIP-1 as a tool for neurodegenerative disease research and diagnosis.

Main Methods:

  • Synthesis of the chemiluminescent amyloid-binding probe (CLIP-1).
  • Evaluation of CLIP-1's chemiluminescence in the presence of aggregated and monomeric Aβ, α-syn, and tau.
  • Assessment of CLIP-1's emission wavelength differences with various amyloid aggregates.
  • Imaging of brain slices from an Alzheimer's disease mouse model.

Main Results:

  • CLIP-1 showed enhanced chemiluminescence with aggregated Aβ, activated by oxygen, with minimal signal for monomeric Aβ.
  • Distinct emission wavelengths were observed for CLIP-1 bound to tau aggregates versus Aβ or α-syn aggregates.
  • Imaging confirmed enhanced chemiluminescence of CLIP-1 in brain slices from an Alzheimer's disease model.

Conclusions:

  • CLIP-1 is a novel chemiluminescent probe effective for detecting key amyloid biomarkers in neurodegenerative diseases.
  • The probe's oxygen-activated mechanism and distinct emission spectra offer advantages over fluorescent probes.
  • CLIP-1 shows potential as a diagnostic tool for amyloid-associated neurodegenerative diseases, particularly for in vivo applications.