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Updated: Jun 15, 2026
Use of Human Perivascular Stem Cells for Bone Regeneration
Published on: May 25, 2012
BMP-2 Enhanced Bone Regeneration Using Clinically Available Grafting Strategies in a Chronic Caprine Tibial Defect
Nicolás S Piuzzi1,2, Viviane Luangphakdy2, Michael S Ramos1
1Department of Orthopaedic Surgery, Cleveland Clinic Foundation, Cleveland, OH.
Objectives:
The aim of this study was to assess bone grafting strategies in the chronic caprine tibial defect (CCTD) model.
Methods:
Design: Preclinical investigation.Setting: Chronic caprine tibial defect model.Patient Selection Criteria: Female Spanish-Boer goats, 4 to 6 years old.Outcome Measures and Comparisons: Primary outcome was total bone volume measured by MicroCT at 12 weeks. Secondary outcomes were radial percent bone volume and moment angle of inertia on MicroCT, and cortical bone bridging on radiograph.
Results:
Animals with MCA + BMP-2 (4542.1 mm 3 , P = 0.026) and MCA + BMP-2+BMA (3633.1 mm 3 , P = 0.006) grafts showed higher median tBV within the central 2.5 cm of the defect compared with animals with MCA + BMA grafts (12.3 mm 3 ). Median tBV within the central 2.5 cm did not differ significantly between animals grafted with ACB (486.8 mm 3 ) and those with MCA + BMP-2 (4542.1 mm 3 ) or MCA + BMA + BMP-2 (3633.1 mm 3 ) grafts. Adding unprocessed BMA to either MCA (MCA: 2.7 mm 3 vs. MCA + BMA: 12.3 mm 3 ) or MCA + BMP-2 (MCA + BMP-2: 4542.1 mm 3 vs. MCA + BMA + BMP-2: 3633.1 mm 3 ) did not significantly increase median tBV within the central 2.5 cm.
Conclusions:
In the CCTD model, BMP-2 had a positive effect on tBV, comparable with and trending greater than ACB. This study supports using a composite of MCA + BMP-2 for large segmental bone defects, whereas the effect of unprocessed BMA requires further evaluation.
