Histological changes in the structural organization of rat kidneys 3 days after administration of Leiurus macroctenus...

Aim: To investigate histological changes in the structural organization of rat kidneys 3 days after administration of Leiurus macroctenus scorpion venom.

Materials and Methods: The venom of Leiurus macroctenus, was administered to rats (0.5 ml of venom solution; 28.8 μg/ml; LD50=0.08 mg/kg). The study used 20 white male laboratory rats weighing 200 g (±10 g). The rats were divided into two groups: control group - 10 rats; experimental group - 10 rats. For microscopic examination, kidney samples from animals of all groups were taken. Histological samples of rat kidneys were stained with hematoxylin and eosin.

Results: Microscopically, on the 3rd day of the experiment after the introduction of Leiurus macroctenus scorpion venom, significant destructive-degenerative changes in all structural components of the kidney were detected. Most renal corpuscles are large with well-defined vascular glomeruli. The endothelium of the capillaries is thinned, destructively changed. Heterogeneity of changes in proximal and distal tubules is manifested.

Conclusions: The indirect effect of the Leiurus macroctenus scorpion venom led to the formation of voluminous inflammatory loci of leukocyte infiltrates in the interstitium and directly in the tubules and renal corpuscles on the 3rd day of the experiment. Significant alterative changes in the renal corpuscles were manifested by their deformation, atrophy, hyperemia and multiple hemorrhages. In the tubular system, pronounced hydropic and protein dystrophy were detected, and in their lumens, voluminous accumulations of cellular detritus were detected.