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Related Concept Videos

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Updated: Apr 30, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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Single-cell profiling reveals pervasive heterogeneity in subcellular RNA localization.

Xiaojuan Fan1, Markus Hafner1

  • 1RNA Molecular Biology Laboratory, National Institute of Arthritis and Musculoskeletal and Skin Disease, Bethesda, MD, USA.

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|April 29, 2026
PubMed
Summary
This summary is machine-generated.

Subcellular RNA localization, a key gene regulator, shows significant cell-to-cell variation. The RNA Localization Profiler (RLP) reveals distinct RNA patterns within cells, uncovering a new layer of cell identity.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Subcellular RNA localization is crucial for gene regulation.
  • Heterogeneity in RNA localization across individual cells is not well understood.

Purpose of the Study:

  • To introduce a novel method, RNA Localization Profiler (RLP), for mapping compartment-specific RNAs in living cells.
  • To investigate subcellular RNA localization heterogeneity and its impact on cell identity.

Main Methods:

  • Developed RNA Localization Profiler (RLP), a proximity-based RNA-editing strategy.
  • Applied single-cell RLP (scRLP) to quantify RNA localization in different cellular compartments (cytoplasm, ER, plasma membrane).

Main Results:

  • RLP identified specific RNA localization programs linked to translation and membrane organization.
  • scRLP revealed significant subcellular RNA localization heterogeneity, with <10% of cytoplasmic RNAs associated with the ER.
  • Spatial RNA patterns were found to be an independent axis of cell-state identity, distinct from gene expression.

Conclusions:

  • Heterogeneous subcellular RNA localization is a fundamental aspect of intracellular organization and cell identity.
  • RLP provides a powerful tool for studying spatial RNA biology in single cells.