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Related Experiment Video

Updated: Apr 30, 2026

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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INDETERMINATE DOMAIN 9 negatively regulates rice crown root development.

Yuanyuan Yang1, Zihao Shi1, Xuan Nie1

  • 1College of Food and Biology, Jingchu University of Technology, Jingmen, Hubei, China.

Frontiers in Plant Science
|April 29, 2026
PubMed
Summary

OsIDD9 negatively regulates rice crown root development. Knockout lines show enhanced growth, while over-expression lines have defects, revealing OsIDD9

Keywords:
OsCRL5OsIDD9OsWOX11crown rootriceuORF

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Area of Science:

  • Plant biology
  • Genetics
  • Molecular biology

Background:

  • Crown root development is crucial for rice plant architecture and nutrient uptake.
  • Plant-specific transcription factors regulate crown root formation, but the role of the IDD family remains unclear.

Purpose of the Study:

  • To elucidate the function of OsIDD9 in rice crown root development.
  • To identify the molecular mechanisms underlying OsIDD9's role.

Main Methods:

  • Genetic analysis of OsIDD9 over-expression and knockout lines.
  • Biochemical and molecular assays to determine gene function and regulation.

Main Results:

  • OsIDD9 acts as a negative regulator of crown root development.
  • OsIDD9 influences downstream genes like OsWOX11 and OsCRL5.
  • OsIDD9 expression is regulated at the translational level by an upstream open reading frame (uORF).

Conclusions:

  • OsIDD9 plays a critical role in rice crown root development through a specific genetic pathway.
  • This study provides insights into the complex interactions of transcription factors in rice root development.