Related Experiment Video

Updated: Jul 18, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

11.2K

Immunoblot-based activity assay for heme-containing histidine kinases

Grant W Larson1, Eaindra Yee1, Ambika Bhagi-Damodaran2

  • 1Department of Chemistry, University of Minnesota, Twin Cities, Minneapolis, MN-55455, USA.

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
|April 29, 2026
PubMed
Summary

No abstract available in PubMed .

Keywords:
AutophosphorylationHemeHistidine kinaseRedox signalingTuberculosis

More Related Videos

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
09:17

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Published on: January 11, 2017

8.2K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.3K

Related Experiment Videos

Last Updated: Jul 18, 2026

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

11.2K
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
09:17

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Published on: January 11, 2017

8.2K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.3K

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Articles linked to this work by shared authors, journal, and citation graph.

Controllable Multihalogenation of a Non-native Substrate by the SyrB2 Iron Halogenase.

ACS catalysis·2026

2-Oxoglutarate Analog-Based Biomolecular Tools for Exploring Structure-Activity Relationships in Nonheme Iron Enzymes.

Chembiochem : a European journal of chemical biology·2025

Rational design of tertiary coordination sphere of a heme-based sensor for two-orders enhanced oxygen affinity.

bioRxiv : the preprint server for biology·2025

Immunoblot-based activity assay for heme-containing histidine kinases.

bioRxiv : the preprint server for biology·2025

Sequential oxidation of L-lysine by a non-heme iron hydroxylase.

Chemical communications (Cambridge, England)·2025

Sequential oxidation of L-lysine by a non-heme iron hydroxylase.

bioRxiv : the preprint server for biology·2025

Identifying the proton donor in electrocatalytic hydrogen evolution by a model hydrogenase.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Electrochemical characterization of C. pasteurianum hydrogenase II and three site-directed variants: catalysis and inhibition.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Insights into the pH-dependent reactivity and substrate induced active-site reorganization of the non- heme iron enzyme CthEgtB.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Lysine or histidine? examining the role of the proximal ligand at the active heme center of cytochrome c nitrite reductase.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Molecular strategies for flux control in an electron bifurcation protein.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Genetic dissection of formate hydrogenlyase-2 function: a metalloenzyme involved in bacterial hydrogen production.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026

Factor XI Inhibition for Ischemic Stroke Secondary Prevention: Pharmacology, Clinical Evidence, and Future Directions.

Journal of clinical pharmacology·2026

Hyaluronic Acid in the Management of Foot and Ankle Pathologies.

Foot & ankle specialist·2026

Audit and Evaluation of the Effect of Fresh Frozen Plasma and Adjunct Pharmacological Agents (Vitamin K1, Prothrombin Complex Concentrate, Tranexamic Acid, and Recombinant Factor VIIa) on Pre- and Post- Transfusion INR in Patients with Coagulopathy: A Prospective Study.

Journal of pharmacy & bioallied sciences·2026

Temporal phosphoproteomics reveals key regulators of procoagulant COAT platelet generation.

Blood vessels, thrombosis & hemostasis·2026

Molecular control of platelet extravasation into tumors and its impact on tumor growth.

Blood vessels, thrombosis & hemostasis·2026

Augmenting hemopexin-mediated heme scavenging mitigates sepsis-induced acute kidney injury in humanized sickle cell mice.

Blood vessels, thrombosis & hemostasis·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us