Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Evolution of enzyme structure.

B S Hartley

    Proceedings of the Royal Society of London. Series B, Biological Sciences
    |September 21, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Enzyme structures reveal evolutionary patterns like divergence and convergence. Experiments on microbial pentitol metabolism provide insights into metabolic pathway evolution using gene analysis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [14] Maleylation of amino groups.

    Methods in enzymology·2012
    Same author

    Glucose isomerase: insights into protein engineering for increased thermostability.

    Biochimica et biophysica acta·2001
    Same author

    The amino acid sequence of ribitol dehydrogenase-F, a mutant enzyme with improved xylitol dehydrogenase activity.

    Journal of protein chemistry·1999
    Same author

    Cultivation of an L-lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle.

    Biotechnology and bioengineering·1994
    Same author

    Arthrobacter D-xylose isomerase: chemical modification of carboxy groups and protein engineering of pH optimum.

    The Biochemical journal·1993
    Same author

    Fermentation of lactose by yeast cells secreting recombinant fungal lactase.

    Applied and environmental microbiology·1993
    Same journal

    Studies on the mechanism of action of picrotoxinin and other convulsants at the crustacean muscle GABA receptor.

    Proceedings of the Royal Society of London. Series B, Biological sciences·2015
    Same journal

    The dynamics of burrowing in Ensis (Bibalvia).

    Proceedings of the Royal Society of London. Series B, Biological sciences·2014
    Same journal

    The release of vasopressin without oxytocin in response to haemorrhage.

    Proceedings of the Royal Society of London. Series B, Biological sciences·2014
    Same journal

    Hypothalamic pathways for independent release of vasopressin and oxytocin.

    Proceedings of the Royal Society of London. Series B, Biological sciences·2014
    Same journal

    Variation in the long-tailed field-mouse (Apodemus sylvaticus (L>)) in the Channel Islands.

    Proceedings of the Royal Society of London. Series B, Biological sciences·2014
    Same journal

    The effect of undernutrition on the postnatal development of the brain and cord in pigs.

    Proceedings of the Royal Society of London. Series B, Biological sciences·2014
    See all related articles

    Area of Science:

    • Biochemistry and Molecular Evolution
    • Enzymology
    • Microbial Genetics

    Background:

    • Three-dimensional enzyme structures provide valuable data for understanding evolutionary processes.
    • Observed similarities in enzyme structures can indicate divergent evolution (e.g., mammalian serine proteases) or convergent evolution (e.g., chymotrypsin and subtilisin).
    • Topological similarities in dehydrogenases present ambiguity, potentially stemming from ancient divergence or inherent chemical constraints on protein architecture.

    Purpose of the Study:

    • To investigate enzyme evolution using experimental approaches.
    • To explore the evolutionary history of metabolic pathways through targeted selective pressure.
    • To gather further experimental evidence supporting hypotheses based on molecular morphology.

    Main Methods:

    Related Experiment Videos

    • Utilizing chemostat cultivation to grow microorganisms on novel substrates, thereby applying selective pressure on specific enzyme activities.
    • Focusing selective pressure on pentitol metabolism pathways.
    • Analyzing the fine structure of genes involved in pentitol metabolism.

    Main Results:

    • Demonstrated the feasibility of using chemostats to direct enzyme evolution.
    • Identified specific evolutionary clues within the gene structures related to pentitol metabolism.
    • Provided experimental support for the link between enzyme structure, function, and evolutionary history.

    Conclusions:

    • Enzyme structure analysis is a powerful tool for inferring evolutionary relationships.
    • Experimental evolution in controlled environments like chemostats can elucidate the development of metabolic pathways.
    • Gene fine structure analysis offers critical insights into the evolutionary trajectory of metabolic processes.