Surgical protocol for precise and high-throughput viral injections in rhesus monkey brain

Viral injections in nonhuman primates allow modification of neurons to investigate the organization and function of neural circuits, their developmental processes, and disease-related mechanisms. Here, we present a protocol for performing precise stereotaxic microinjections in the macaque brain. We describe steps for pre-surgical imaging, target planning, viral injections, and perioperative care. We then detail procedures for tissue extraction and slice electrophysiology experiments in multiple subcortical and cortical regions. For complete details on the use and execution of this protocol, please refer to Furlanis et al.¹.