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circRNA circ-ZEB1 promotes gallbladder carcinomas progression by regulating the miR-144-3p/ZEB2 axis
Luoshun Huang1, Yang Zhang1, Fan Yang1
1Department of Hepatobiliary Surgery, Taizhou Municipal Hospital (Taizhou University Affiliated Municipal Hospital), School of Medicine, Taizhou University, Taizhou, Zhejiang, China.
Neoplasma
|April 29, 2026
Summary
Circular RNA circ-ZEB1 promotes gallbladder cancer (GBC) growth by sponging miR-144-3p to upregulate ZEB2. This circ-ZEB1/miR-144-3p/ZEB2 pathway is a potential therapeutic target for GBC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gallbladder cancer (GBC) is an aggressive biliary system tumor.
- Circular RNAs (circRNAs) are implicated in GBC tumorigenesis.
- The role of novel circRNAs, like circ-ZEB1, in GBC requires further investigation.
Purpose of the Study:
- To investigate the function and mechanism of circ-ZEB1 in GBC.
- To explore the relationship between circ-ZEB1, miR-144-3p, and ZEB2 in GBC cells.
Main Methods:
- RT-qPCR and western blot to detect gene expression.
- Cell proliferation, migration, and colony formation assays.
- Subcellular localization, xenograft experiments, and mechanistic studies.
Main Results:
- Circ-ZEB1 was upregulated and located in the cytoplasm of GBC cells.
- Circ-ZEB1 knockdown inhibited GBC cell proliferation, migration, and tumor growth in vivo.
- Circ-ZEB1 acted as a sponge for miR-144-3p, targeting ZEB2, and this axis was crucial for GBC progression.
Conclusions:
- The circ-ZEB1/miR-144-3p/ZEB2 axis plays a critical role in gallbladder cancer advancement.
- Circ-ZEB1 may represent a novel therapeutic target for GBC treatment.
