Related Experiment Video
Updated: May 1, 2026

Quantitative Assessment Protocol for Facial Soft Tissue Volumetric Changes with Stereophotogrammetry
Published on: December 9, 2025
Validating the AFA3D forensic method using live CT data: A multiregional comparison of approximated and original
Renitta Rajan Thottungal1, Pierre Guyomarc'h2, Ján Dupej3
1Laboratory of 3D Imaging and Analytical Methods, Department of Anthropology and Human Genetics, Faculty of Science, Charles University, Prague, Czech Republic; Department of Anatomy, Third Faculty of Medicine, Charles University, Ruská 2411, Prague 100 00, Czech Republic.
Abstract:
Facial approximation aids identification of unknown individuals in forensic and anthropological contexts. Digital approximation methods estimate Facial Soft‑Tissue Thickness (FSTT) and facial shape from virtual skulls, which are meant to lower subjectivity. Yet substantial variability persists in predicted outcomes, particularly in the nasal and lower facial regions. AFA3D (Anthropological Facial Approximation in Three Dimensions), developed by Guyomarc'h et al. (2014) from French data, generates facial predictions using statistical shape modelling, FSTT‑based warping, and iterative algorithms. Earlier studies reported moderate error in the mouth and smaller errors in nasal and orbital areas, but its broader performance remains insufficiently evaluated. This study assesses AFA3D by comparing approximated faces with original facial meshes from 40 CT-scans, 10 each from Czech, Slovak, Egyptian, and French samples. Geometric morphometric comparison was conducted using Morphome3cs II. Across samples, 75.9-84.2% of facial surfaces fell within ±2.5 mm deviation. Systematic regional errors were observed in the nose, lips, chin, cheeks, and upper face, with males generally showing greater localised discrepancies than females. These patterns correspond to anatomical regions with limited skeletal constraint and to sex‑linked cranial structural differences, as observed in previous approximation validations. Overall, AFA3D produces predictions with consistent regional error patterns, underscoring the need for more detailed mapping of local deviations, better modelling of posture‑related influences, and continued refinement of automated approximation methods to strengthen forensic reliability.

