Related Experiment Video
Updated: May 1, 2026

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
Published on: April 28, 2022
Dynamics studies of side-chains and hydration shells in amyloid-β fibrils using deuterium and oxygen-17 solid-state
Liliya Vugmeyster1, Dmitry Ostrovsky2
1Department of Chemistry, University of Colorado at Denver, Denver CO, United States.
Abstract:
This review focuses on deuterium and oxygen-17 solid-state NMR methods for studies of dynamics in amyloid-beta fibrils in rehydrated lyophilized states. For both nuclei NMR spectroscopic properties are dominated by the quadrupolar interaction. Deuterium NMR methods for studies of side-chain dynamics cover line shape analysis, longitudinal Zeeman and Quadrupolar order relaxation, transverse relaxation using Qudrupolar Carr Purcell Meiboom Gill scheme real-time detection, rotating frame relaxation (including off-resonance and quadrupolar order locking), and Chemical Exchange Saturation Transfer methods. Examples are chosen based on the results obtained for native and post-translationally modified fibrils under static and magic-angle spinning conditions. Oxygen-17 laboratory and rotating frame relaxation methods focusing on detection of the central transition under static conditions are presented for hydration shell dynamics in pyroglutateme-3 modified fibrils. Experimental methodology is supplemented with description of explicit motional modeling approaches to arrive at quantitative description of dynamical modes and their corresponding parameters. Special emphasis is placed on the complementarity of different approaches to obtain a fuller picture of motions.
More Related Videos
14:55Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
Published on: September 17, 2017
08:40Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
Published on: June 23, 2022
Related Concept Videos
¹H NMR of Labile Protons: Deuterium (²H) Substitution
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
Amyloid Fibrils
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
Amyloid Fibrils
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
¹H NMR: Long-Range Coupling
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene...