Burdock leaf polysaccharide as a clean-label bio-preservative for meat: Structural characterization, multifaceted
Yong-Xin Li1, Chan Zhang1, Linchong Hui1
1JSNU-UWEC Joint Laboratory of Jiangsu Province Colleges and Universities, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Abstract:
The development of natural preservatives from agricultural by-products is essential for sustainable food systems. This study valorized burdock leaves by extracting a bioactive polysaccharide with yield of 6.422%. A burdock leaf polysaccharide (BPS) was identified as an acidic heteropolysaccharide (molecular weight 1.616 × 105 Da) with a triple-helix structure, mainly composed of galactose and arabinose. It exhibited strong antioxidant activity and notable antibacterial efficacy against Escherichia coli and Staphylococcus aureus (minimum inhibitory concentration (MIC50) = 25 mg/mL). Mechanistic analyses revealed that BPS disrupted membrane integrity, elevated reactive oxygen species (ROS) levels, and induced DNA damage. When applied as a beef coating and stored in a refrigerator at 4 °C, BPS extended shelf-life from 6 to over 12 days by preserving color, limiting weight loss and pH increase, and suppressing bacterial growth and total volatile basic nitrogen (TVB-N) accumulation. These findings highlight BPS as a promising clean-label preservative derived from agricultural waste.
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