Antagonistic modulation of myosin conformational dynamics by sanshool and smoke-derived aldehydes: A molecular basis...

This study explored how smoke aldehydes (furfural and 5-methylfurfural (5-MF)) and sanshool from Zanthoxylum L. regulate myosin dynamics to affect antioxidant activity, peroxidation, and heterocyclic amines (HAs) formation. Results showed that two aldehydes reduced protein antioxidant activity, accelerated peroxidation, and promoted the formation of HAs. However, sanshool exhibited a protective effect, inhibiting peroxidation and HAs generation. Critically, in co-exposure scenarios, sanshool effectively counteracted the pro-HAs effects of the aldehydes, demonstrating its dominant inhibitory role. The three molecules bind to myosin through a static quenching mechanism, inducing a reduction in α-helix content. Molecular dynamics simulations revealed that furfural attenuated the structural alterations triggered by sanshool, which provided a structural foundation for its antagonistic effect on the HA-inhibitory activity of sanshool. This study provides new insights into how interactions between hazardous and functional food molecules regulate protein conformation and influence food safety, providing a scientific basis for developing novel HAs inhibition technologies.