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Contamination potential of non-resorbable membranes intended for exposure in the oral cavity during regenerative
Lucas Trevisan Suzzin1, Artur Villas Boas Weckwerth2, Verci Alves de Oliveira3
1Department of Periodontics and Implant Dentistry, Ingá University Center, Rod PR 317, 6114 , Maringá, PR, 87035-510, Brazil.
Background:
Some non-resorbable membranes are indicated to be left exposed in the oral cavity during regeneration procedures. Given the frequency or indication of exposure of these membranes in the oral cavity, it is worth studying their pattern of contamination, which may lead to undesirable clinical results.
Purpose:
This study aimed to evaluate and compare microbial contamination among non-resorbable membranes intended for exposure in the oral cavity during regenerative procedures.
Methods:
Samples of non-resorbable membranes were used: Bone Heal® (BH), Cytoplast Txt-200® (CT), Lumina PTFE® (L), and Surgitime Titanium Seal® (STS). Fifty-six samples were divided into groups: group S (control) - sterile membranes; group C - membranes contaminated with human oral biofilm. These samples were analyzed by counting colony-forming units and scanning electron microscopy/energy dispersive spectroscopy. One-way ANOVA and Tukey's tests were used to compare colony-forming units among the contaminated groups. The paired t-test was used to compare the colony-forming units between the two groups of each membrane, with a significance level of 5%.
Results:
The membranes showed similar contamination values, with the BH membrane being the least contaminated and the L membrane the most contaminated (p = 0.005). The membranes presented surfaces with different patterns and designs, also showing different levels of contamination that seem to be related to surface characteristics and composition.
Conclusion:
All studied membranes intended for exposure in the oral cavity became contaminated, showing different patterns, with membrane BH exhibiting the lowest contamination.
Insights
All non-resorbable membranes exposed during oral regeneration procedures showed microbial contamination. Bone Heal (BH) membranes had the lowest contamination levels, while Lumina PTFE (L) membranes had the highest.
Area of Science:
- Biomaterials Science
- Oral Microbiology
- Regenerative Dentistry
Background:
- Non-resorbable membranes are used in oral regeneration procedures and may be exposed.
- Exposure can lead to microbial contamination, potentially impacting clinical outcomes.
Purpose of the Study:
- To evaluate and compare microbial contamination on non-resorbable membranes intended for oral exposure.
- To investigate the relationship between membrane surface characteristics and contamination levels.
Main Methods:
- Four types of non-resorbable membranes (Bone Heal, Cytoplast Txt-200, Lumina PTFE, Surgitime Titanium Seal) were tested.
- Samples were divided into sterile (control) and orally biofilm-contaminated groups.
- Microbial contamination was assessed using colony-forming unit counts and scanning electron microscopy/energy dispersive spectroscopy.
Main Results:
- All tested membranes showed microbial contamination after exposure to oral biofilm.
- Bone Heal (BH) membranes exhibited the lowest contamination, while Lumina PTFE (L) membranes showed the highest (p=0.005).
- Contamination levels appeared related to the membranes' surface patterns, design, and composition.
Conclusions:
- Non-resorbable membranes intended for oral exposure are susceptible to microbial contamination.
- Significant differences in contamination patterns exist among membrane types, with BH showing the least contamination.
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