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Related Experiment Video

Updated: May 1, 2026

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction

Published on: November 1, 2018

Dengue Virus Capsid Protein Interaction With Nucleic Acids.

Nelly M Silva^1,2, Ana S Martins^1,2, Nina E Karguth^1,2

¹GIMM - Gulbenkian Institute for Molecular Medicine, Lisbon, Portugal.

Biofactors (Oxford, England)

|April 30, 2026

Summary

This summary is machine-generated.

Dengue virus (DENV) capsid protein interacts with the viral genome. This interaction involves molecular condensation, potentially through liquid-liquid phase separation, aiding DENV replication.

Keywords:

capsid protein dengue virus liquid–liquid phase separation nucleic acids protein‐DNA interaction

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Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

Area of Science:

Virology
Molecular Biology
Biophysics

Background:

Dengue virus (DENV) transmission is increasing globally, with expanded mosquito vector ranges.
DENV outbreaks are occurring in new regions like Europe.
The DENV capsid (C) protein is crucial for viral replication, binding host lipids and the viral genome.

Purpose of the Study:

To investigate the biophysical mechanism of Dengue virus capsid protein association with the viral genome.
To understand how this interaction contributes to viral encapsidation and replication.

Main Methods:

Biophysical characterization of Dengue virus C protein interaction with single-stranded DNA (ssDNA) mimicking genomic regions.
Utilized fluorescence intensity and lifetime measurements.
Analyzed changes in protein secondary structure.

Main Results:

Observed a decrease in fluorescence intensity and lifetime upon DENV C protein binding to ssDNA.
Detected alterations in the protein's secondary structure.
Results suggest molecular condensation and liquid-liquid phase separation.

Conclusions:

The Dengue virus capsid protein interacts with the viral genome through a process involving molecular condensation.
Liquid-liquid phase separation may play a role in forming DENV C-nucleic acid complexes.
Understanding this interaction is key for developing novel antiviral therapies.