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Influenza

Influenza

Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...

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Related Experiment Video

Updated: May 1, 2026

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Published on: April 15, 2017

Identification of Influenza H1N1 Endonuclease Inhibitor via Virtual Screening and In Vitro Validation.

¹Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Madinah, Saudi Arabia.

Medicinal Chemistry (Shariqah (United Arab Emirates))

|April 30, 2026

Summary

Researchers identified a novel triazole-pyridine derivative as a potent inhibitor of the H1N1 influenza virus polymerase acidic endonuclease. This compound shows promising antiviral activity and a favorable safety profile for developing new influenza therapeutics.

Keywords:

Influenza drug discovery endonuclease inhibitor molecular docking virtual screening

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Last Updated: May 1, 2026

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

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Published on: April 15, 2017

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Published on: October 11, 2013

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

Area of Science:

Virology
Medicinal Chemistry
Computational Chemistry

Background:

The H1N1 influenza pandemic poses a significant global health threat, necessitating the development of novel antiviral drugs due to emerging resistant strains.
The influenza polymerase acidic (PA) endonuclease is a critical target for antiviral development, essential for viral replication.

Purpose of the Study:

To identify novel inhibitors of the H1N1 PA endonuclease using a virtual fragment-based screening approach.
To validate the inhibitory activity and cytotoxicity of identified compounds against H1N1 influenza.

Main Methods:

Virtual screening of a fragment-based chemical library against the H1N1 PA endonuclease active site.
Experimental validation using crystal violet and MTT assays to determine antiviral activity and cytotoxicity in MDCK cells.

Main Results:

A novel triazole-pyridine derivative was identified as a potent inhibitor of the H1N1 PA endonuclease.
The compound demonstrated significant antiviral activity with an IC50 of 1.392 μM/mL and a favorable selectivity index (CC2⁽ = 495 μmol/mL).
Binding affinity was correlated with specific residue interactions within the PA active site.

Conclusions:

The identified triazole-pyridine derivative is a promising lead compound for developing new H1N1 influenza inhibitors.
Virtual fragment-based screening is an effective method for discovering novel antiviral agents targeting viral enzymes.
This study provides a valuable direction for next-generation influenza therapeutics with improved safety profiles.