Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fibroblast-Selective Lipid Nanoparticles for Tumor Stromal Transfection.

Molecular pharmaceutics·2026

Same author

Inter-annual and regional variability of agronomic and grain quality traits of major Korean wheat cultivars under multi-environmental conditions.

Journal of the science of food and agriculture·2026

Same author

A Dual-Imaging Fluorescent and Iodinated Thermosensitive Hydrogel for Image-Guided Surgery of Pulmonary Nodules.

ACS applied bio materials·2026

Same author

Cholera toxin as a model AB<sub>5</sub> toxin: functional significance of B-subunit pentamerization in GM1 recognition, pathogenicity, and delivery efficiency.

Archives of toxicology·2026

Same author

Supramolecular Chiral Assembly of Open-Shell Quinoids With Chiral Additives and Their Spin-Dependent Transport in Magneto Field-Effect Transistors.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Reassessing Parity-Related Variation in Female Body Morphology: A Matched Analysis in a Contemporary Population.

American journal of human biology : the official journal of the Human Biology Council·2026

Search research articles

Related Experiment Video

Updated: May 1, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Surfactant-Triggered Signal Enhancement for Lateral Flow Immunoassay.

Ji-Ho Park¹, Jinhee Park², Yunseul Kim^3,4

¹Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.

|April 30, 2026

Summary

This study presents a simple, automated method to significantly boost the sensitivity of lateral flow assays (LFAs). The new platform enhances point-of-care testing (POCT) for biomarkers like cardiac troponin I with improved accuracy and low cost.

Keywords:

automated signal enhancement lateral flow assay myocardial infraction one step signal enhancement point-of-care testing

More Related Videos

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Related Experiment Videos

Last Updated: May 1, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

Area of Science:

Biomedical Engineering
Analytical Chemistry
Nanotechnology

Background:

Lateral flow assays (LFAs) are crucial point-of-care testing (POCT) tools due to their affordability and ease of use, particularly in resource-limited settings.
A significant limitation of LFAs is their insufficient sensitivity for detecting low concentrations of biomarkers.
Existing signal enhancement methods often involve complex procedures or multiple steps, hindering practical application.

Purpose of the Study:

To develop a novel, one-step, automated signal enhancement platform for LFAs.
To improve the sensitivity of LFAs without increasing complexity or cost.
To demonstrate the platform's compatibility and effectiveness with a clinically relevant biomarker.

Main Methods:

A surfactant-triggered release system was engineered using the hydrophobic properties of office paper to automate the in situ growth of gold nanoparticles.
The release rate of the enhancement solution was controlled by adjusting surfactant concentration and paper layering, enabling tunable, delayed release.
The platform was tested for the detection of cardiac troponin I, a biomarker for myocardial infarction.

Main Results:

The automated platform achieved a 157-fold increase in sensitivity for cardiac troponin I detection compared to unenhanced LFAs.
A limit of detection of 2.42 pg mL⁻¹ was achieved within 20 minutes.
The material cost for the enhancement was less than $0.5 per test, demonstrating economic viability.

Conclusions:

The developed one-step, automated signal enhancement platform offers a low-cost, user-friendly, and highly sensitive solution for LFAs.
This technology significantly overcomes the sensitivity limitations of traditional LFAs.
The platform shows substantial potential for widespread adoption in universal point-of-care testing applications.