Related Experiment Video
Updated: May 1, 2026
04:20
Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
Published on: February 9, 2024
1.6K
GO-Y078 Triggers Program Cell Death in Human Cervical Cancer Cells Via MAPK Activation-Dependent Apoptotic Caspases
Chung-Yuan Lee1,2, Po-Hui Wang3,4, Yi-Hsien Hsieh3,4
1Chiayi Chang Gung Memorial Hospital, Chiayi, Taiwan.
Integrative Cancer Therapies
|April 30, 2026
Summary
GO-Y078, a curcumin analog, induces programmed cell death (apoptosis) in human cervical cancer cells. This compound activates the MAPK pathway, leading to caspase activation and potential therapeutic use.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Apoptosis is crucial for removing damaged cells.
- GO-Y078, a curcumin analog, shows anti-cancer effects but its mechanism in cervical cancer is unclear.
Purpose of the Study:
- To investigate the anti-cancer mechanism of GO-Y078 in human cervical cancer cells.
- To elucidate the signaling pathways involved in GO-Y078-induced apoptosis.
Main Methods:
- Assessed cell viability, chromatin condensation, and apoptosis in SiHa and HeLa cells treated with GO-Y078.
- Analyzed the involvement of Poly (ADP-ribose) polymerase (PARP) and caspases (-9, -8, -3).
- Investigated the role of the mitogen-activated protein kinase (MAPK) pathway and specific inhibitors (U0126, SB203580).
Main Results:
- GO-Y078 reduced cervical cancer cell viability and induced apoptosis.
- Activation of PARP and caspases (-9, -8, -3) was observed.
- GO-Y078 elevated MAPK pathway phosphorylation; inhibiting ERK or p38 reduced caspase activation.
Conclusions:
- GO-Y078 induces apoptotic cell death in cervical cancer.
- The mechanism involves MAPK pathway activation and subsequent caspase cascade.
- GO-Y078 shows potential as a therapeutic agent for cervical cancer.
Related Concept Videos
01:07MAPK Signaling Cascades
7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
01:31The Intrinsic Apoptotic Pathway
6.2K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
01:17The Extrinsic Apoptotic Pathway
6.2K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.2K
01:06Induced Pluripotent Stem Cells
4.8K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.8K
01:24Caspases
8.7K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
8.7K
02:38Mitogens and the Cell Cycle
6.3K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.3K