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Magnetic ionic liquid-functionalized graphene for DNA extraction and authentication of beef products
Xin Tang1, Yao Chen2, Xueqin Ding3
1School of Life Sciences, Shanghai University, Shanghai 200444, China; School of Life Sciences, Hunan Normal University, Changsha 410081, China.
Journal of Chromatography. A
|April 30, 2026
Summary
A novel graphene-based magnetic adsorbent simplifies DNA extraction for food authentication. This efficient method aids in identifying meat species and ensuring food supply chain authenticity.
Area of Science:
- Food Science
- Analytical Chemistry
- Materials Science
Background:
- Efficient DNA extraction is crucial for food authentication, especially for identifying meat species.
- Conventional magnetic solid-phase extraction methods are complex and require multistep synthesis.
- A simplified, effective DNA extraction material is needed for practical food analysis.
Purpose of the Study:
- To develop a novel magnetic adsorbent for simplified and efficient DNA extraction.
- To establish a rapid, one-step DNA extraction method for food authentication.
- To validate the method's efficacy in identifying meat species in food products.
Main Methods:
- Synthesis of a magnetic ionic liquid-functionalized graphene adsorbent (G-MIL).
- Development of a one-step DNA extraction protocol using vortex-assisted dispersion and magnetic separation.
- Optimization of extraction parameters and assessment of selectivity against interfering substances.
Main Results:
- The G-MIL adsorbent integrates magnetic and functional properties, simplifying synthesis.
- The one-step method efficiently isolates, enriches, and purifies DNA without centrifugation.
- The method showed high repeatability (RSD = 2.15%) and selectivity for DNA.
- Successful application in authenticating beef products, distinguishing pure from adulterated samples.
Conclusions:
- The developed G-MIL adsorbent offers a novel and efficient solution for DNA extraction.
- The rapid, one-step method simplifies DNA isolation for food authentication.
- This approach enhances food traceability and ensures authenticity in the food supply chain.
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