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  3. Switchable Dna-scaffolded Multivalent Aptamer Through Stimuli-responsive Plug-and-play Modules For Protein Regulation.
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  3. Switchable Dna-scaffolded Multivalent Aptamer Through Stimuli-responsive Plug-and-play Modules For Protein Regulation.

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Switchable DNA-Scaffolded Multivalent Aptamer through Stimuli-Responsive Plug-and-Play Modules for Protein

Kaiqi Xu1,2, Ji Zhao3, Guang Hu4

  • 1Department of Chemistry, University of Science & Technology of China, Hefei, Anhui 230026, China.

ACS Applied Materials & Interfaces
|April 30, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Researchers developed a switchable multivalent system using DNA tiles and aptamers to control thrombin activity. This programmable platform offers dynamic, reversible regulation in response to multiple stimuli like UV light.

Keywords:
DNA self-assemblyMultivalent aptamerPlug-and-play modulesProtein regulationStimuli-responsive switches

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Area of Science:

  • Biomolecular Engineering
  • Synthetic Biology
  • Nanotechnology

Background:

  • Multivalent molecular interactions are crucial for biological functions, driving interest in multivalent materials for biosensing and medicine.
  • Reversible switching between low-valency and multivalency is essential for functional regulation but challenging to achieve with multiple stimuli.
  • Existing designs struggle to integrate multi-stimuli responsiveness for dynamic valency switching within a single system.

Purpose of the Study:

  • To demonstrate a novel switchable multivalent system for dynamic regulation of thrombin activity.
  • To introduce stimuli-responsive modules for plug-and-play switching in a DNA aptamer system.
  • To explore multi-stimuli responsiveness for precise control over molecular interactions.

Main Methods:

  • Constructed a multivalent system using thrombin aptamer-decorated DNA tile assembly.
  • Integrated plug-and-play modules responsive to UV light, APE1, and RNase H as orthogonal switches.
  • Developed three-layer switching cascades activated by one, two, or three stimuli to modulate thrombin activity.

Main Results:

  • Successfully demonstrated a switchable multivalent system capable of reversible valency control.
  • Achieved dynamic regulation of thrombin activity through stimuli-responsive DNA tile assembly.
  • Engineered multi-stimuli responsive cascades and a tristate circuit for complex control logic.

Conclusions:

  • The developed switchable DNA scaffold provides a versatile, modular platform for programmable multivalent regulation.
  • This approach enables precise control over molecular interactions and biological activity.
  • Potential applications include precision medicine, advanced biosensing, and novel biomaterials.