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Fine mapping of the TdHLS gene controlling hairy leaf sheath from wild emmer wheat
Nannan Liu1, Wenxin Wei1, Junna Sun1
1State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.
Key Message:
The TdHLS gene controlling hairy leaf sheath derived from wild emmer wheat was fine-mapped to a ~2.3 Mb physical interval on chromosome 4BL based on the Zavitan (WEW_v1.0) reference genome. Trichomes on leaf sheaths serve as specialized epidermal structures that form a protective barrier for plants. Wild emmer wheat (WEW), a tetraploid progenitor of common wheat, represents a valuable genetic resource for wheat breeding programs. This study reported the fine mapping of TdHLS, a gene controlling hairy leaf sheath that was introgressed from WEW. Genetic mapping by F2 and F3 (0T267/Han 87-1*2 and 0T267/AK58*2) individuals revealed this gene was delimited in a 2.3 Mb region on chromosome 4BL based on the WEW reference genome (Zavitan v1.0), and this region shows observed recombination suppression. Transcriptomic and sequence analyses identified four expressed genes containing nonsynonymous mutations between hairy and glabrous leaf sheath lines, within the 28 annotated genes located in the target interval. Among them TRIDC4BG062310 (encoding an oxysterol-binding protein-related protein) exhibited differential expression patterns, suggesting its potential role in trichome development. Collinearity analysis indicated orthologous hairy leaf sheath loci in WEW (Zavitan v1.0), barley (Morex v1.0) and Aegilops tauschii (AL8/78 v4.0), indicating the presence of evolutionarily conserved homologous genes governing this morphological characteristic. Introgression lines demonstrated successful trait incorporation without affecting plant architecture and grain characteristics. This study establishes a foundation for cloning the TdHLS gene and provides a valuable morphological marker for wheat breeding.
