Autostaining Immune Multiplex Immunohistochemistry Panels and Imaging Analysis

Immune cells often require several markers for positive identification. While flow-cytometry can provide detailed multi-parameter cell identification it does not retain the cell localization. Multiplex immunohistochemistry provides a valuable technique for cell profiling with localization in the tissue microenvironment. In this study, we present the staining procedures for human immune-cell multiplex panels, employing tyramide signal amplification-based immunohistochemistry on an autostainer. Additionally, we explore the imaging analysis techniques applied to the stained tissues. These panels enable the visualization of immune cell markers on formalin-fixed paraffin-embedded tissues.