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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: May 2, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Fully Integrated Sample Preparation-Based Spatial Proteomic Analysis.

Qian Kong^1,2, Xi Wang³, Ruijun Tian^4,5

¹Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen, China.

Methods in Molecular Biology (Clifton, N.J.)

|April 30, 2026

Summary

This summary is machine-generated.

Spatial proteomics utilizes laser microdissection (LMD) and advanced mass spectrometry to analyze complex biological tissues. This powerful strategy deciphers the tissue microenvironment for a deeper understanding of cellular machinery.

Keywords:

LC-MS LMD SISPROT Spatial proteomics

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Area of Science:

Proteomics
Molecular Biology
Biotechnology

Background:

Understanding the tissue microenvironment is crucial for elucidating biological machinery.
Spatial proteomics is emerging as a key technology for this purpose.

Purpose of the Study:

To describe protocols for spatial proteomics using laser microdissection (LMD) and liquid chromatography-mass spectrometry (LC-MS).
To detail methods for comprehensive analysis of the tissue microenvironment.

Main Methods:

Tissue slice preparation and staining.
Laser microdissection (LMD) for histological region collection.
Simple and integrated spintip-based proteomics technology (SISPROT) for sample preparation.
High-sensitive liquid chromatography-mass spectrometry (LC-MS) analysis.

Main Results:

The described protocols enable detailed spatial proteomics analysis.
Integration of LMD and SISPROT with LC-MS provides a powerful strategy for tissue microenvironment studies.

Conclusions:

This chapter provides a comprehensive guide to spatial proteomics protocols.
The described methodology facilitates in-depth elucidation of biological machinery within complex tissues.