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Updated: May 2, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
Published on: July 6, 2022
Fully Integrated Sample Preparation-Based Spatial Proteomic Analysis
Qian Kong1,2, Xi Wang3, Ruijun Tian4,5
1Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen, China.
Abstract:
To decipher the complex tissue microenvironment for comprehensively elucidating the biological machinery, spatial proteomics is becoming popular. Laser microdissection (LMD) technology combined with integrated sample preparation and high-sensitive liquid chromatography-mass spectrometry (LC-MS) is the most powerful strategy. In this chapter, we describe the protocols for tissue slice preparation and staining, LMD-based histological regions collection, simple and integrated spintip-based proteomics technology (SISPROT) based sample preparation, and highly sensitive LC-MS analysis.

