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Related Experiment Video

Updated: Jul 8, 2026

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion

Published on: September 25, 2019

Detailed Protocol for Expansion of Large Tissue Samples for Expansion Microscopy.

Vibeke S Dam¹, Amalie M Grønning¹, Benjamin B Green¹

¹Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|April 30, 2026

Summary

This summary is machine-generated.

Expansion microscopy (ExM) physically enlarges biological samples to improve optical resolution. This study details an optimized ExM protocol for rat kidney inner medulla tissue biopsies, adaptable for other tissues.

Keywords:

Expansion microscopy (ExM)Kidney Optical resolution Protocol Tissue biopsies

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Area of Science:

Biotechnology
Microscopy
Cell Biology

Background:

Expansion microscopy (ExM) is a key technique for enhancing optical resolution in biological imaging.
ExM achieves higher resolution by physically expanding specimens, increasing distances between fluorophores and improving sample transparency.

Purpose of the Study:

To develop and optimize an Expansion Microscopy (ExM) protocol specifically for small tissue biopsies.
To adapt ExM for imaging the inner medulla of perfusion-fixed rat kidneys.

Main Methods:

The study optimized an ExM protocol for tissue samples measuring 2 mm in diameter and 1 mm in thickness.
The protocol was applied to perfusion-fixed rat kidney inner medulla samples.

Main Results:

The optimized ExM protocol successfully enlarged the biological samples.
The enlargement improved optical resolution and sample transparency, facilitating detailed imaging.

Conclusions:

A robust ExM protocol has been established for rat kidney inner medulla biopsies.
This protocol shows potential for application to other tissue types with minor adjustments.