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Updated: Jul 8, 2026

Nanoscopic Imaging of Human Tissue Sections via Physical and Isotropic Expansion
Published on: September 25, 2019
Detailed Protocol for Expansion of Large Tissue Samples for Expansion Microscopy
Vibeke S Dam1, Amalie M Grønning1, Benjamin B Green1
1Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
None:
Expansion microscopy (ExM) is a powerful technique that enhances optical resolution by physically enlarging biological samples. The enlargement increases the distances between fluorophores and makes the sample transparent. Here, we present an ExM protocol optimized for tissue biopsies with 2 mm diameter and 1 mm thickness from inner medulla from perfusion-fixed rat kidneys. Besides inner medulla kidney samples, the protocol may be utilized for other tissue types with minor modifications.

