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Abnormal Sperm Morphology in Lodpr/lncRNA-HSVIII Knockout Mice with Advancing Age
Pummarin Tippramuan1,2, Luo Yujia2, Yui Satoh2
1Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand.
Reproductive Sciences (Thousand Oaks, Calif.)
|April 30, 2026
Summary
The long noncoding RNA Lodpr/lncRNA-HSVIII influences sperm morphology and testicular tissue. Knockout mice showed age-dependent changes, including altered sperm tails and increased vascularization.
Area of Science:
- Reproductive Biology
- Genetics
- Molecular Biology
Background:
- The function of the mouse testis-specific long noncoding RNA, Lodpr/lncRNA-HSVIII, is unknown.
- This RNA is expressed in germ and Leydig cells.
Purpose of the Study:
- To investigate the physiological role of Lodpr/lncRNA-HSVIII.
- To characterize the phenotype of knockout mice.
Main Methods:
- CRISPR/Cas9 technology was used to generate knockout mice.
- Phenotypic traits were assessed at 2 and 6 months of age.
- Histological examination, testicular analysis, and sperm analysis were performed.
Main Results:
- Lodpr/lncRNA-HSVIII knockout mice showed increased testicular vascularization and altered seminiferous tubule stages.
- At 6 months, knockout mice exhibited increased Sertoli cells, germ cell degeneration, and abnormal sperm with bent tails.
- Increased extravascular leukocytes were observed in the cauda epididymis of 6-month-old knockout mice.
Conclusions:
- Lodpr/lncRNA-HSVIII modulates sperm morphology, testicular tissue, and epididymis.
- The effects of Lodpr/lncRNA-HSVIII deficiency become more pronounced with age.
