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Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples Using Data
Niels van Boxtel1, Heli Nygren1, Tuulikki Seppänen-Laakso1
1VTT Technical Research Centre of Finland Ltd, Espoo, Finland.
None:
In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For the characterization of lipids, data dependent acquisition (DDA) analyses are carried out utilizing a high-resolution quadrupole-time-of-flight (Q-ToF) instrument. Throughput of the method is over 100 samples/day. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
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