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Updated: May 2, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Published on: November 10, 2021
Analytical Grade Purification of Phycocyanin from Cyanobacteria
Mahammed Ilyas Khazi1,2, Zeliha Demirel3, Fakhra Liaqat4
1Department of Biology, Faculty of Arts and Sciences, Bursa Uludag University, Bursa, Turkey. ilyaskhazi@uludag.edu.tr.
Abstract:
Phycocyanin is a blue-colored pigment-protein complex that exhibits numerous biofunctions such as anti-inflammation, antioxidation, antitumor, neuroprotective effect, and immunological enhancement. Purified phycocyanin has pharmaceutical and nutraceutical applications. In addition, as a nontoxic and non-carcinogenic natural coloring agent, phycocyanin has many applications in the food and cosmetic industries. This chapter describes a protocol for extraction and analytical grade purification of phycocyanin from cyanobacteria. The purification steps include (1) extraction of phycocyanin from biomass, (2) ammonium sulfate precipitation of phycocyanin and dialysis, and (3) purification of phycocyanin by gel filtration and ion-exchange chromatography.
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