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Phytoplankton Pigment Analysis by High Performance Liquid Chromatography.

Thomas Rohrlack¹

¹Faculty of Environmental Sciences and Natural Resource Management, Ås, Norway. Thomas.Rohrlack@nmbu.no.

Methods in Molecular Biology (Clifton, N.J.)

|April 30, 2026

Summary

Phytoplankton pigments in water and sediment are key to understanding aquatic ecosystems. This study details a high-performance liquid chromatography method for analyzing these crucial biological markers.

Keywords:

Carotenoids Chlorophylls Extraction HPLC Phytoplankton Pigment analysis

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Area of Science:

Environmental science
Analytical chemistry
Marine biology

Background:

Pigments serve as vital chemotaxonomic markers for phytoplankton.
Sedimentary pigments act as biological archives, preserving historical aquatic environment data.
Accurate pigment analysis is essential for ecological and historical water body studies.

Purpose of the Study:

To present protocols for extracting pigments from water and sediment samples.
To describe a comprehensive method for phytoplankton pigment analysis.
To enable the separation, identification, and quantification of major phytoplankton pigments.

Main Methods:

Pigment extraction from water and sediment samples.
High-performance liquid chromatography with diode array detection (HPLC-DAD).
Single-run analysis for multiple pigment identification and quantification.

Main Results:

Established protocols for efficient pigment extraction.
Developed and validated an HPLC-DAD method for phytoplankton pigment analysis.
Demonstrated the capability to separate, identify, and quantify major pigments in a single analytical run.

Conclusions:

The presented protocols and HPLC-DAD method provide a robust approach for phytoplankton pigment analysis.
This method enhances the ability to determine phytoplankton composition and reconstruct past aquatic conditions.
The findings support the use of sedimentary pigments as reliable biological archives for water body histories.