Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

928
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
928
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

8.6K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
8.6K
The Nitrogen Cycle01:49

The Nitrogen Cycle

46.5K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
46.5K
Gravimetry: Inorganic And Organic Precipitating Agents00:49

Gravimetry: Inorganic And Organic Precipitating Agents

5.9K
In gravimetry, the precipitant is chosen carefully to obtain a pure solid that can be easily filtered. Common inorganic precipitants can be used to determine several cations and anions. In some cases, the formation of the same precipitate can be used to determine the cation and the anion. For example, the reaction of barium and chromate ions to give barium chromate is used to determine both barium and chromate. However, precipitates such as hydroxides, oxalates, and metal ammonium phosphates...
5.9K
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

1.3K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.3K
Key Elements for Plant Nutrition02:35

Key Elements for Plant Nutrition

17.9K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
17.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Basic Methods for Isolating and Culturing Microalgae.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Measurement of Fluorescence for Monitoring Algal Growth and Health.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Large Scale Cultivation of Microalgae in Open, Closed, and Hybrid Systems.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Growing Algae in Biofilms: Principles and Emerging Opportunities.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Determining Inorganic and Organic Carbon.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Total Nitrogen Determination by a Spectrophotometric Method.

Methods in molecular biology (Clifton, N.J.)·2026

Related Experiment Video

Updated: May 2, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
08:44

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis

Published on: May 10, 2020

6.3K

Determining Inorganic and Organic Nitrogen.

Jaana Koistinen1, Mervi Sjöblom2, Kristian Spilling3,4

  • 1Tvärminne Zoological Station, University of Helsinki, Hanko, Finland. jaana.koistinen@helsinki.fi.

Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2026
PubMed
Summary

Accurate measurement of nitrogen (N) is vital for algal cultivation. This study details methods for quantifying dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON) to support algal growth.

Keywords:
AmmoniumDissolved organic nitrogenNitrateNutrient uptakeParticulate organic nitrogen

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

5.5K
Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.9K

Related Experiment Videos

Last Updated: May 2, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
08:44

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis

Published on: May 10, 2020

6.3K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

5.5K
Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.9K

Area of Science:

  • Aquatic biology
  • Biogeochemistry
  • Nutrient cycling

Background:

  • Nitrogen is essential for algal growth, forming key cellular components like proteins.
  • Understanding nitrogen availability is critical for optimizing algal cultivation.
  • Accurate quantification of different nitrogen forms is necessary for ecological and agricultural applications.

Purpose of the Study:

  • To present reliable methods for determining dissolved inorganic nitrogen (DIN), dissolved organic nitrogen (DON), and particulate organic nitrogen (PON).
  • To provide a comprehensive guide for researchers and cultivators on nitrogen analysis in aquatic systems.

Main Methods:

  • Dissolved inorganic nitrogen (DIN) determined using established colorimetric techniques.
  • Dissolved organic nitrogen (DON) and particulate organic nitrogen (PON) quantified via filtration followed by high-temperature catalytic oxidation.
  • Integration of these methods for a complete nitrogen pool assessment.

Main Results:

  • Validated colorimetric methods for DIN analysis provide accurate and reproducible results.
  • High-temperature catalytic oxidation effectively quantifies DON and PON fractions.
  • The combined methodology offers a robust approach to nitrogen speciation in algal cultures.

Conclusions:

  • The presented methods enable precise determination of all major nitrogen pools in aquatic environments.
  • Accurate nitrogen measurements are crucial for advancing algal cultivation and understanding aquatic ecosystems.
  • These techniques contribute to improved management of nitrogen-limited systems for biomass production.