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Updated: May 2, 2026
Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Qualitative and flow cytometric evaluations of goat's frozen semen in Mito-TEMPO supplemented plant-based extender
Fatemeh Zarei1, Nader Asadzadeh2, Hassan Khamisabadi3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Background:
Cryopreservation of buck spermatozoa is an efficient strategy to improve qualified sperm cells for artificial insemination; however, this process potentially decreases the qualitative characteristics of post-thawed sperm. Therefore, this research aimed to evaluate the influence of the mitochondria-targeted antioxidant "Mito-TEMPO" on buck sperm quality during the cryopreservation process in a plant-based extender.
Results:
This study indicated that the dosages 10 and 100 µM Mito-TEMPO presented higher (P ≤ 0.05) percentages of total motility, progressive motility, membrane integrity, mitochondrial activity, acrosome integrity, and viability while showing lower (P ≤ 0.05) late apoptosis, DNA fragmentation, reactive oxygen species (ROS) concentration, and lipid peroxidation compared to the control group. Different treatments of Mito-TEMPO indicated no effect (P > 0.05) on sperm cells with abnormal morphology.
Conclusions:
It can be concluded that this mitochondria-targeted antioxidant may be able to preserve the qualitative parameters in the post-thawed buck spermatozoa.
