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Updated: May 13, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

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Published on: March 3, 2010

Standardized Workflows for Time-Resolved Singlet Oxygen Quantification in Aqueous Systems.

Heryerli Fernandez¹, Helena C Junqueira^2,3, Lucas F S Hess²

¹Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Departamento de Química, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CCT La Plata-CONICET, Diagonal 113 y 64, S/N, 1900 La Plata, Argentina.

|May 1, 2026

Summary

This summary is machine-generated.

This study introduces standardized workflows and the SOLIS framework for accurately quantifying singlet oxygen (¹O₂) in aqueous systems. The method improves reproducibility and reliability of photophysical parameter determination.

Keywords:

liposomes phosphorescence lifetime quantum yield singlet oxygen

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Area of Science:

Photochemistry
Physical Chemistry
Biophysical Chemistry

Background:

Direct time-resolved phosphorescence detection is crucial for quantifying singlet oxygen (¹O₂).
Challenges in aqueous environments include rapid solvent quenching and coupled decays of ¹O₂ and photosensitizer triplets.
Existing methods struggle with reliable photophysical parameter determination due to these complexities.

Purpose of the Study:

To establish standardized workflows for acquiring and analyzing ¹O₂ kinetics in aqueous systems.
To develop and implement the open-source SOLIS computational framework for kinetic modeling.
To enable accurate quantification of photophysical parameters and oxidative processes.

Main Methods:

Utilized direct time-resolved phosphorescence detection.
Applied homogeneous and diffusion-coupled kinetic models via the SOLIS framework.
Implemented structured artifact and model-consistency checks with objective fit-quality criteria.

Main Results:

The SOLIS framework provides reliable and self-consistent photophysical parameters (quantum yields, lifetimes) for ¹O₂.
The workflow mitigates inconsistencies from subjective fitting window selection.
Quantified lipid-to-water signal contributions in heterogeneous systems.

Conclusions:

The developed workflow enhances reproducibility in quantifying ¹O₂ kinetics.
This approach supports quantitative evaluation of oxidative processes in diverse fields.
Standardized protocols facilitate reliable photophysical parameter determination in aqueous environments.