Suppression of salt-enhanced apoplastic flow by salicylic acid in rice

Salinity enhances apoplastic flow, resulting in an increment of Na⁺ uptake and a lower K⁺/Na⁺ ratio. Salicylic acid (SA) plays an important role in improving salinity tolerance in plants. The effect of exogenous SA on apoplastic flow in salt-treated rice seedlings was studied using an apoplastic tracer, 8-hydroxy-1,3,6-pyrenetrisulphonic acid (PTS) in light. Application of NaCl at 25 mM to the hydroponic solution significantly increased PTS uptake, while 25 mM NaCl did not affect seedling growth. Application of 25 mM NaNO₃ increased PTS uptake to the same degree. Salinity significantly increased sodium (Na⁺) content but had no significant effect on potassium (K⁺) content, resulting in a lower K⁺/Na⁺ ratio. The application of SA at 0.05 mM and 0.1 mM to the hydroponic solution reduced Na-enhanced PTS uptake. Salicylic acid at 0.05 mM and 0.1 mM significantly reduced Na⁺ content and slightly increased K⁺ content in the shoots of rice seedlings, resulting in a higher K⁺/Na⁺ ratio. However, SA at up to 0.1 mM did not increase SA contents in shoots under salt stress. These results suggest that exogenous SA reduces Na⁺ uptake by suppressing Na⁺-enhanced apoplastic flow in rice seedlings. These findings provide insight into modulation of Na⁺ transport pathways from roots to shoots by SA and may allow us to utilize brackish water for rice cultivation and to improve salt-tolerant rice through suppression of salt-enhanced apoplastic flow by chemicals such as salicylic acid.

The online version contains supplementary material available at 10.1007/s12298-026-01733-3.