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Updated: May 2, 2026

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii
Published on: May 6, 2022
Culture method for promoting efficient ciliary growth in the Chlamydomonas pf23 mutant defective in dynein
Ryosuke Yamamoto1, Takahide Kon1
1Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka, Ōsaka, Japan.
Abstract:
Chlamydomonas reinhardtii is an ideal model organism for studying the cytoplasmic preassembly of ciliary dyneins. However, under normal liquid-culture conditions, Chlamydomonas preassembly-deficient mutants often show no cilia or a small number of ciliated cells (i.e., low ciliation ratio), which hinders further analysis of both ciliary dyneins and the phenotypes of these mutants. In this brief report, we present a modified culture method for one of the Chlamydomonas preassembly-deficient mutants, pf23 . This method enables researchers to obtain enough pf23 cilia for small-scale biochemical, biophysical, structural and phenotypic analyses.

