Related Experiment Video

Updated: May 2, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

LINC01420 as a oncogenic lncRNA: Expression signatures, functional roles, and prognostic significance in

Ning Tang¹, Rongyao Zhou², Rui Sun¹

¹Division of Hepatobiliary and Transplantation Surgery, Department of General Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, No. 321, Zhongshan Road, Gulou District, Nanjing, 210008, Jiangsu, China.

Irish Journal of Medical Science

|May 1, 2026

Abstract

Background:

Cholangiocarcinoma (CCA) is a commonly occurred malignancy, and has a worrying survival rate. This study aimed to investigate the relationship between long non-coding RNA LINC01420 and CCA overall survival, and explore the biological function and mechanisms of LINC01420 in CCA progression.

Methods:

LINC01420 and miR-320a levels were measured by quantitative real-time PCR. Kaplan-Meier survival curve was performed to analyze the correlation between LINC01420 expression and overall survival. CCK-8 and Transwell assay evaluated CCA cell proliferation, migration, and invasion, respectively. The interaction between LINC01420 and miR-320a by dual-luciferase reporter assay. Bioinformatics analysis predicted miR-320a target genes, related functions and pathway information.

Results:

LINC01420 expression was significantly increased in CCA tumor tissues and cells. LINC01420 overexpression was significantly correlated with larger tumor size, lymph node metastasis and advanced TNM stage. LINC01420 was predicted worse overall survival in CCA patients. Knockdown of LINC01420 inhibited CCA cell proliferation, migration and invasion, and these effects could be abolished by the reduction of miR-320a, which was determined as a target of LINC01420 in CCA.

Conclusion:

LINC01420 downregulation inhibited tumor progression by acting as a ceRNA of miR-320a in CCA cells. The LINC01420/miR-320a axis may provide novel biomarkers and targets for CCA therapy.

Keywords:

Cholangiocarcinoma LINC01420 Prognosis Proliferation ceRNAs miR-320a

Related Concept Videos

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Loss of Tumor Suppressor Gene Functions

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...

Cancer-Critical Genes I: Proto-oncogenes

Cancer-Critical Genes I: Proto-oncogenes

Articles linked to this work by shared authors, journal, and citation graph.

Calcium/calmodulin-dependent protein kinase II links ER stress with Fas and mitochondrial apoptosis pathways.

The Journal of clinical investigation·2009

Cripto-1 overexpression is involved in the tumorigenesis of nasopharyngeal carcinoma.

BMC cancer·2009

Range of motion and orientation of the lumbar facet joints in vivo.

Spine·2009

[Silencing of COX-2 in nasopharyngeal carcinoma cells with a shRNAmir lentivirus vector].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2009

The risk of melamine-induced nephrolithiasis in young children starts at a lower intake level than recommended by the WHO.

Pediatric nephrology (Berlin, Germany)·2009

Adult scoliosis in patients over sixty-five years of age: outcomes of operative versus nonoperative treatment at a minimum two-year follow-up.

Spine·2009

See all related articles