Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spontaneous and Induced Mutations01:30

Spontaneous and Induced Mutations

3.2K
Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).
3.2K
Mismatch Repair01:20

Mismatch Repair

5.4K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.4K
Mismatch Repair01:36

Mismatch Repair

38.1K
Overview
38.1K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

2.0K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
2.0K
Mutations01:39

Mutations

66.8K
Overview
66.8K
Mutations01:35

Mutations

31.2K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
31.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi‑scale immune and spatial profiling of sclerosing adenosis and surrounding breast tissue identifies an immune‑cold field phenotype associated with breast cancer risk.

Breast cancer research : BCR·2026
Same author

PRSS23 promotes ovarian cancer peritoneal dissemination independent of protease activity.

The Journal of biological chemistry·2026
Same author

Antagonistic SMAD2/3 control of TIMP-1, VEGF-A, and hypoxia signaling in myofibroblasts shapes histotype-specific angiogenesis in lung cancer.

Cell death & disease·2026
Same author

Epidermal Growth Factor Receptor/KIT-Linked Proliferative Bias in Normal Breast Lobules from Matched Non-Hispanic Black and White Women Is Rapidly Reversible by Receptor Tyrosine Kinase Inhibition.

The American journal of pathology·2026
Same author

Postpartum breast cancer: evidence for a distinct phenotype.

Journal of the National Cancer Institute·2026
Same author

Tissue Microarray-Based Digital Spatial Profiling of Benign Breast Lobules and Breast Cancers: Feasibility, Biological Coherence, and Cross-Platform Benchmarks.

Cancers·2025

Related Experiment Video

Updated: May 3, 2026

The Lambda Select cII Mutation Detection System
07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

7.5K

When tissue tension creates a mutagenic niche.

Nicole Cruz-Reyes1, Derek C Radisky1

  • 1Department of Cancer Biology, Mayo Clinic, Jacksonville, FL 32224, USA.

Cancer Cell
|May 1, 2026
PubMed
Summary

Fibrotic tissue tension creates a mutagenic niche in cancer. This stiffness activates epithelial STAT3 and drives lipid peroxidation, damaging DNA in fibrotic tumors and dense breast tissue.

Area of Science:

  • Oncology
  • Cancer Biology
  • Biophysics

Background:

  • Fibrotic tissue is common in tumors and dense breast tissue.
  • Tissue stiffness can influence cellular behavior and disease progression.

Purpose of the Study:

  • To investigate how fibrotic tissue tension contributes to a mutagenic microenvironment.
  • To identify the molecular mechanisms linking tissue stiffness to DNA damage in epithelial cells.

Main Methods:

  • Analysis of fibrotic tumor and mammographically dense breast tissue.
  • Investigating the role of Signal Transducer and Activator of Transcription 3 (STAT3) activation.
  • Assessing NADPH oxidase (NOX)-dependent lipid peroxidation and its products.

Main Results:

More Related Videos

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

2.8K
An Introduction to Worm Lab: from Culturing Worms to Mutagenesis
10:44

An Introduction to Worm Lab: from Culturing Worms to Mutagenesis

Published on: January 11, 2011

34.8K

Related Experiment Videos

Last Updated: May 3, 2026

The Lambda Select cII Mutation Detection System
07:08

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

7.5K
Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

2.8K
An Introduction to Worm Lab: from Culturing Worms to Mutagenesis
10:44

An Introduction to Worm Lab: from Culturing Worms to Mutagenesis

Published on: January 11, 2011

34.8K
  • Fibrotic tissue tension establishes a mechanically organized mutagenic niche.
  • A stiff stroma activates epithelial STAT3 and recruits macrophages.
  • NOX-dependent lipid peroxidation generates diffusible aldehydes that damage epithelial DNA.

Conclusions:

  • Mechanical forces in fibrotic tissue promote tumorigenesis through DNA damage.
  • Targeting fibrotic pathways may offer new therapeutic strategies for cancer treatment.