Related Experiment Video
Updated: May 3, 2026

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
Multisegment C-to-N Sequential Ser/Thr Ligation Using Mmoc-Protected N-Terminal Serine/Threonine Peptide
Jiahui Tang1, Qingyao Shu1, Jun Wang1
1School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Key Laboratory of Animal Source of Anhui Province, Hefei University of Technology, Hefei 230009, China.
Abstract:
Multisegment Ser/Thr ligation (STL) is an important technique for the chemical synthesis of proteins. The Fmoc group is currently widely used in multisegment STL to temporarily protect the reactive Ser or Thr residue at the N-terminus of the salicylaldehyde ester segment(s). However, the alkaline conditions required for Fmoc removal are incompatible with base-sensitive post-translational modifications and can readily induce aspartimide formation. Here, we report the Mmoc group-based multisegment STL. The Mmoc protection group can be rapidly removed under trifluoroacetic acid (TFA) and NH4I conditions, and this new strategy has been proven to efficiently synthesize proteins containing O-acetylation as well as Asp-Gly sequences.
More Related Videos
Related Concept Videos
Phosphodiester Linkages
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

