Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Psychiatric comorbidities and treatment patterns in epilepsy: A retrospective review from a tertiary neuropsychiatric clinic.

Indian journal of psychiatry·2026
Same author

Mitochondrial dysfunction in schizophrenia and its modulation by atypical antipsychotic drugs: A randomized controlled trial.

Journal of psychopharmacology (Oxford, England)·2026
Same author

Pulse‑level characterization of low monitor unit deliveries on a modern linear accelerator using a plastic scintillation detector.

Journal of applied clinical medical physics·2026
Same author

Design and synthesis of <i>N</i>-3-substituted quinazolinone derivatives as anticancer agents targeting EGFR.

RSC medicinal chemistry·2026
Same author

Quantitative perfusion imaging from non-contrast micro-ct for pulmonary embolism evaluation in preclinical models.

Physics in medicine and biology·2026
Same author

Design, structural stability, and performance of a cesium magneto-optical trap with large optical access using additively manufactured magnetic field coil mounts.

The Review of scientific instruments·2026

Related Experiment Video

Updated: May 4, 2026

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

9.2K

Image-Guided In Vivo Tracking of Splenocyte Dynamics Using Superparamagnetic Iron Oxide-Based Nanoparticles in a

Archana Mishra1, Dominic Karl M Bolinas1,2, Poonam Yadav1

  • 1Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, Houston, Texas 77030, United States.

ACS Applied Materials & Interfaces
|May 2, 2026
PubMed
Summary
This summary is machine-generated.

Researchers tracked immune cells from the spleen to tumors using novel nanoparticles. Splenocyte-mediated delivery, driven by macrophages, shows promise for targeted cancer immunotherapy.

Keywords:
cancerin vivo trackingmagnetic resonance imaging (MRI)splenocytessuperparamagnetic iron oxide (SPIO) nanoparticlestargeted delivery

More Related Videos

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
10:03

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model

Published on: November 21, 2019

8.9K
Tracking Mouse Bone Marrow Monocytes In Vivo
12:08

Tracking Mouse Bone Marrow Monocytes In Vivo

Published on: February 27, 2015

9.3K

Related Experiment Videos

Last Updated: May 4, 2026

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
09:06

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Published on: March 31, 2008

9.2K
Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model
10:03

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model

Published on: November 21, 2019

8.9K
Tracking Mouse Bone Marrow Monocytes In Vivo
12:08

Tracking Mouse Bone Marrow Monocytes In Vivo

Published on: February 27, 2015

9.3K

Area of Science:

  • Immunology
  • Nanotechnology
  • Biomedical Engineering

Background:

  • The spleen is a reservoir for immune cells crucial in inflammation and cancer.
  • Understanding immune cell migration from the spleen to tumors is vital for cancer biology and immunotherapy.
  • Current methods for tracking these dynamics are limited.

Purpose of the Study:

  • To develop and validate a noninvasive method for tracking splenocyte migration in vivo.
  • To investigate the potential of splenocytes as carriers for targeted nanoparticle delivery to tumors.
  • To elucidate the role of specific immune cell subtypes in this trafficking process.

Main Methods:

  • Splenocytes were labeled with superparamagnetic iron oxide (SPIO) nanoparticles conjugated with protamine-indocyanine green (SPIOpICG).
  • Nanoparticle characterization included transmission electron microscopy and hydrodynamic size analysis.
  • In vivo tracking utilized MRI in tumor-bearing rat models, followed by histological and flow cytometry analysis of tumor tissues.

Main Results:

  • SPIOpICG-labeled splenocytes demonstrated high viability and uptake, confirming biocompatibility.
  • MRI revealed significant splenocyte migration to the liver, spleen, and importantly, the tumor site.
  • Flow cytometry confirmed higher accumulation of SPIOpICG-positive cells in tumors following SPIOpICG-splenocyte injection compared to free SPIOpICG.
  • Phenotypic analysis identified M2 macrophages as the predominant SPIOpICG-positive cell population within tumors.

Conclusions:

  • Splenocyte-mediated delivery, primarily via macrophage trafficking, enables targeted nanoparticle accumulation at tumor sites.
  • This study validates splenocytes as effective natural carriers for targeted nanoparticle delivery.
  • The developed SPIOpICG nanoconjugate platform offers a promising tool for studying immune cell dynamics and advancing immunotherapies.