Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

2.5K
2.5K
Regulated Protein Degradation02:58

Regulated Protein Degradation

6.6K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
6.6K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.2K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

3.5K
3.5K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

11.9K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
11.9K
The Proteasome01:13

The Proteasome

1.6K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Akkermansia muciniphila-derived postbiotics reprogram immune balance to combat sepsis via the IDO1/Kyn/AhR metabolic axis.

Journal of advanced research·2026
Same author

Electric-Field Sensor Based on Multilayer-MoS<sub>2</sub>/Multilayer-Graphene Heterostructure with Built-In Tensile Strain.

ACS applied materials & interfaces·2026
Same author

Artemisinin increases susceptibility to ferroptosis of fibroblast-like synoviocytes in rheumatoid arthritis.

Arthritis research & therapy·2026
Same author

Inhibiting hepatocytes MAGL alleviates osteoporosis caused by high-fat diet-induced liver fibrosis in male mice.

Journal of the Endocrine Society·2026
Same author

Crosstalk between bone and vasculature within bone.

Fundamental research·2026
Same author

Fluoxetine-associated adverse event signals with a focus on seizure: A study based on the FAERS database, network pharmacology, and molecular docking.

Progress in neuro-psychopharmacology & biological psychiatry·2026

Related Experiment Video

Updated: May 4, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

790

JOSD2 deubiquitinating enzyme: Structure, function, and potential as a therapeutic target.

Zefen Li1, Jiali Hu2, Ling Wang2

  • 1First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China; Department of Pathology, Affiliated Hospital of Jiujiang University, Jiujiang 332000, China.

Cellular Signalling
|May 2, 2026
PubMed
Summary

Josephin domain-containing 2 (JOSD2) is a deubiquitinase regulating cellular signaling. Aberrant JOSD2 activity links to diseases like cancer, cardiovascular disorders, and inflammatory bowel disease, presenting therapeutic opportunities.

Keywords:
Deubiquitinating enzymesInhibitorsJOSD2Therapeutic strategyTumorigenesis

More Related Videos

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

9.3K
Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
07:05

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

1.1K

Related Experiment Videos

Last Updated: May 4, 2026

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

790
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

9.3K
Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay
07:05

Measuring Enzymatic Activity of Neurodevelopmental Disorder-Associated Deubiquitylating Enzymes via an In Vitro Ubiquitin Chain Cleavage Assay

Published on: September 27, 2024

1.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Signaling

Background:

  • Ubiquitination is a key post-translational modification regulating cellular signaling.
  • Josephin domain-containing 2 (JOSD2) is a deubiquitinase that removes ubiquitin, impacting proteostasis and signaling.
  • Dysregulated JOSD2 activity is implicated in various pathological conditions.

Purpose of the Study:

  • To review JOSD2's role as a regulatory node in ubiquitin-dependent signaling.
  • To discuss JOSD2 dysregulation in malignancies and other diseases.
  • To highlight JOSD2's potential as a biomarker and therapeutic target.

Main Methods:

  • Literature review of JOSD2's functions and dysregulation.
  • Analysis of JOSD2's role in cancer, cardiovascular disorders, and inflammatory bowel disease.
  • Identification of knowledge gaps and translational opportunities.

Main Results:

  • JOSD2 regulates signaling through mechanisms including metabolic rewiring and oncogenic signaling circuits.
  • JOSD2 dysregulation contributes to therapeutic resistance in cancer.
  • JOSD2 exhibits context-dependent pathogenic or protective roles in non-cancerous diseases.

Conclusions:

  • JOSD2 is a pleiotropic, context-dependent deubiquitinase with significant roles in cellular signaling.
  • Further research is needed to fully map JOSD2 substrates and tissue-specific functions.
  • JOSD2 holds translational potential as a biomarker and therapeutic target.