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Bacteriophage interference in Bacillus subtilis 168.

R E Yasbin, A T Ganesan, F E Young

    Journal of Virology
    |April 1, 1974
    PubMed
    Summary
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    Bacillus subtilis strains lysogenic for bacteriophage SPO2 prevent the growth of unrelated bacteriophages phi1 and phi105. Infection by bacteriophage phi1 halts host DNA and RNA synthesis, leading to cell death.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Lysogeny, where a bacteriophage integrates into the host genome, can confer resistance to superinfection.
    • Bacteriophage SPO2 is a temperate bacteriophage that establishes lysogeny in Bacillus subtilis.
    • Bacteriophage phi1 and phi105 are unrelated to SPO2 and infect Bacillus subtilis.

    Purpose of the Study:

    • To investigate the interference of Bacillus subtilis lysogenic for bacteriophage SPO2 against unrelated bacteriophages phi1 and phi105.
    • To elucidate the mechanisms underlying bacteriophage interference in lysogenic bacteria.

    Main Methods:

    • Bacterial and bacteriophage culture techniques.
    • Infection assays with bacteriophages phi1 and phi105 on Bacillus subtilis lysogenic for SPO2.

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  • Analysis of host DNA and RNA synthesis following bacteriophage phi1 infection.

    • Main Results:

    • Bacillus subtilis lysogenic for bacteriophage SPO2 exhibited significant inhibition of bacteriophage phi1 development.
    • Infection of lysogenic cells by bacteriophage phi1 led to the termination of host DNA and RNA synthesis and subsequent cell death.
    • Bacteriophage SPO2 also prevented the production of bacteriophage phi105 in infected Bacillus subtilis.

    Conclusions:

    • Lysogeny for temperate bacteriophage SPO2 provides robust protection against superinfection by unrelated bacteriophages.
    • The interference mechanism against bacteriophage phi1 involves the disruption of essential host macromolecular synthesis.
    • Further research is needed to fully delineate the molecular mechanisms of bacteriophage interference.