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Updated: May 5, 2026

Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos
Published on: May 12, 2020
Adjuvant multisite bacteriophage cocktail administration as salvage treatment in a patient with a multidrug-resistant
Aurore Denis1, Tristan Ferry2, Franck Jegoux3
1Infectious Diseases Department and Intensive Care Unit, Pontchaillou University Hospital, Rennes, France.
Background:
Bone infection due to multidrug-resistant (MDR) Pseudomonas aeruginosa is associated with poor outcomes, especially high risk of relapses. Adjunctive multisite phage therapy can be used as a salvage treatment in relapsing cases.
Case Presentation:
We treated a 37-year-old man with MDR P. aeruginosa frontal osteitis and a large frontal fistula, who experienced three relapses, using personalized multisite phage therapy combined with surgery and prolonged antibiotics (ceftolozane/tazobactam). The multisite phage administration included in situ instillation of the manufactured phage cocktail during surgery, followed by daily intravenous infusion and nebulization for 7 days after surgery. No adverse events were reported during treatment. At the 2-year follow-up, the patient had no relapse without any suppressive treatment.
Conclusion:
Multisite phage administration is a personalized treatment strategy that can prevent relapse or the emergence of additional antimicrobial resistance in complex cases, such as relapsing MDR P. aeruginosa osteitis.
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